biostudies-literatureUnknown9(4)Feser RMicroRNA (miRNA/miR) 526 b- and miR655-overexpressed tumor cell-free secretions regulate the breast cancer tumor microenvironment (TME) by promoting tumor-associated angiogenesis, oxidative stress, and hypoxic responses. Additionally, premature miRNA (pri-miR526b and pri-miR655) are established breast cancer blood biomarkers. However, the mechanisms of how these miRNAs regulate the TME has yet to be investigated. Mass spectrometry analysis of miRNA-overexpressed cell lines MCF7-miR526b, MCF7-miR655, and miRNA-low MCF7-Mock cell-free secretomes identified 34 differentially expressed proteins coded by eight genes. In both miRNA-high cell secretomes, four markers are upregulated: <i>YWHAB</i>, <i>SFN</i>, <i>TXNDC12</i>, and <i>MYL6B,</i> and four are downregulated: <i>PEA15</i>, <i>PRDX4</i>Heliyone15421https://www.ebi.ac.uk/biostudies/studies/S-EPMC10148110biostudies-literatureBreast cancer cell secretome analysis to decipher miRNA regulating the tumor microenvironment and discover potential biomarkers.PMC10148110Feser RNault BChen VCOpperman RMMajumder MMaiti SfalseBreast cancer cell secretome analysis to decipher miRNA regulating the tumor microenvironment and discover potential biomarkers.MicroRNA (miRNA/miR) 526 b- and miR655-overexpressed tumor cell-free secretions regulate the breast cancer tumor microenvironment (TME) by promoting tumor-associated angiogenesis, oxidative stress, and hypoxic responses. Additionally, premature miRNA (pri-miR526b and pri-miR655) are established breast cancer blood biomarkers. However, the mechanisms of how these miRNAs regulate the TME has yet to be investigated. Mass spectrometry analysis of miRNA-overexpressed cell lines MCF7-miR526b, MCF7-miR655, and miRNA-low MCF7-Mock cell-free secretomes identified 34 differentially expressed proteins coded by eight genes. In both miRNA-high cell secretomes, four markers are upregulated: <i>YWHAB</i>, <i>SFN</i>, <i>TXNDC12</i>, and <i>MYL6B,</i> and four are downregulated: <i>PEA15</i>, <i>PRDX4</i>2023-01-01T00:00:00Z2023 Apr2026-04-08T14:18:00.832Z2025-04-04T14:18:31.102ZS-EPMC101481103712831810.1016/j.heliyon.2023.e15421