biostudies-literatureNishii KSumitomo FoundationSibbald TrustDarwin Tree of Life ProjectBiotechnology and Biological Sciences Research CouncilJapan Society for the Promotion of Science41https://www.ebi.ac.uk/biostudies/studies/S-EPMC10148486biostudies-literatureUnknown19(1)<h4>Background</h4>PacBio HiFi sequencing provides highly accurate long-read sequencing datasets which are of great advantage for whole genome sequencing projects. One limitation of the method is the requirement for high quality, high molecular weight input DNA. This can be particularly challenging for plants that frequently contain common and species-specific secondary metabolites, which often interfere with downstream processes. Cape Primroses (genus Streptocarpus), are some of these recalcitrant plants and are selected here as material to develop a high quality, high molecular weight DNA extraction protocol for long read genome sequencing.<h4>Results</h4>We developed a DNA extraction method for PacBio HiFi sequencing for Streptocarpus grandis and Streptocarpus kentaniensis. A CTAB lysisPlant methodsA high quality, high molecular weight DNA extraction method for PacBio HiFi genome sequencing of recalcitrant plants.PMC10148486BB/X019683/117020418K063752018#1815K18593Forrest LLHoward CFoster RGHart MLMoller MKelso NBarber SNishii KfalseA high quality, high molecular weight DNA extraction method for PacBio HiFi genome sequencing of recalcitrant plants.<h4>Background</h4>PacBio HiFi sequencing provides highly accurate long-read sequencing datasets which are of great advantage for whole genome sequencing projects. One limitation of the method is the requirement for high quality, high molecular weight input DNA. This can be particularly challenging for plants that frequently contain common and species-specific secondary metabolites, which often interfere with downstream processes. Cape Primroses (genus Streptocarpus), are some of these recalcitrant plants and are selected here as material to develop a high quality, high molecular weight DNA extraction protocol for long read genome sequencing.<h4>Results</h4>We developed a DNA extraction method for PacBio HiFi sequencing for Streptocarpus grandis and Streptocarpus kentaniensis. A CTAB lysis2023-01-01T00:00:00Z2023 Apr2026-04-30T12:28:40.473Z2025-04-05T22:43:34.633ZS-EPMC101484863712060110.1186/s13007-023-01009-x