biostudies-literatureLi HNatural Science Foundation of ChinaZhejiang Key R&amp;D ProgramNational Key R&amp;D Program of China, Stem Cell and Translation ResearchZhejiang Provincial Natural Science Foundation of ChinaZhejiang Province Science Foundation for Distinguished Young Scholars117https://www.ebi.ac.uk/biostudies/studies/S-EPMC10150485biostudies-literatureUnknown14(1)<h4>Background</h4>In vertebrates, hematopoietic stem and progenitor cells (HSPCs) emerge from hemogenic endothelium in the floor of the dorsal aorta and subsequently migrate to secondary niches where they expand and differentiate into committed lineages. Glia maturation factor γ (gmfg) is a key regulator of actin dynamics that was shown to be highly expressed in hematopoietic tissue. Our goal is to investigate the role and mechanism of gmfg in embryonic HSPC development.<h4>Methods</h4>In-depth bioinformatics analysis of our published RNA-seq data identified gmfg as a cogent candidate gene implicated in HSPC development. Loss and gain-of-function strategies were applied to study the biological function of gmfg. Whole-mount in situ hybridization, confocal microscopy, flow cytometry, and weStem cell research & therapyGlia maturation factor-γ is required for initiation and maintenance of hematopoietic stem and progenitor cells.PMC101504858187008091949115LY21H0800042020C03G20135862018YFA01093008200017781730008LR19H0800012019C03016Shan WLi HLuo QTie RCai SHuang HQian PZeng XMeng YXu YfalseGlia maturation factor-γ is required for initiation and maintenance of hematopoietic stem and progenitor cells.<h4>Background</h4>In vertebrates, hematopoietic stem and progenitor cells (HSPCs) emerge from hemogenic endothelium in the floor of the dorsal aorta and subsequently migrate to secondary niches where they expand and differentiate into committed lineages. Glia maturation factor γ (gmfg) is a key regulator of actin dynamics that was shown to be highly expressed in hematopoietic tissue. Our goal is to investigate the role and mechanism of gmfg in embryonic HSPC development.<h4>Methods</h4>In-depth bioinformatics analysis of our published RNA-seq data identified gmfg as a cogent candidate gene implicated in HSPC development. Loss and gain-of-function strategies were applied to study the biological function of gmfg. Whole-mount in situ hybridization, confocal microscopy, flow cytometry, and we2023-01-01T00:00:00Z2023 Apr2026-05-29T03:05:38.731Z2025-02-19T02:38:42.916ZS-EPMC101504853712201410.1186/s13287-023-03328-1