{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Legaria AA"],"funding":["American Heart Association","U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke","NIDDK NIH HHS","Howard Hughes Medical Institute","NINDS NIH HHS","WUSTL | McDonnell Center for Systems Neuroscience","U.S. Department of Health & Human Services | National Institutes of Health"],"pagination":["1124-1128"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10152879"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(9)"],"pubmed_abstract":["Fiber photometry enables recording of population neuronal calcium dynamics in awake mice. While the popularity of fiber photometry has grown in recent years, it remains unclear whether photometry reflects changes in action potential firing (that is, 'spiking') or other changes in neuronal calcium. In microscope-based calcium imaging, optical and analytical approaches can help differentiate somatic from neuropil calcium. However, these approaches cannot be readily applied to fiber photometry. As such, it remains unclear whether the fiber photometry signal reflects changes in somatic calcium, changes in nonsomatic calcium or a combination of the two. Here, using simultaneous in vivo extracellular electrophysiology and fiber photometry, along with in vivo endoscopic one-photon and two-photon calcium imaging, we determined that the striatal fiber photometry does not reflect spiking-related changes in calcium and instead primarily reflects nonsomatic changes in calcium."],"journal":["Nature neuroscience"],"pubmed_title":["Fiber photometry in striatum reflects primarily nonsomatic changes in calcium."],"pmcid":["PMC10152879"],"funding_grant_id":["R35 NS097306","F32 DK126355","916267","P30 DK020579","R01NS122840","R01 NS122840","P30 DK056341","P60 DK020579","DK020579","DK056341","Hanna H. Gray Fellowship","3R35NS097306-04S1","DK126355"],"pubmed_authors":["Parker JG","Kravitz AV","Matikainen-Ankney BA","Legaria AA","Yang B","Licholai JA","Ahanonu B"],"additional_accession":[]},"is_claimable":false,"name":"Fiber photometry in striatum reflects primarily nonsomatic changes in calcium.","description":"Fiber photometry enables recording of population neuronal calcium dynamics in awake mice. While the popularity of fiber photometry has grown in recent years, it remains unclear whether photometry reflects changes in action potential firing (that is, 'spiking') or other changes in neuronal calcium. In microscope-based calcium imaging, optical and analytical approaches can help differentiate somatic from neuropil calcium. However, these approaches cannot be readily applied to fiber photometry. As such, it remains unclear whether the fiber photometry signal reflects changes in somatic calcium, changes in nonsomatic calcium or a combination of the two. Here, using simultaneous in vivo extracellular electrophysiology and fiber photometry, along with in vivo endoscopic one-photon and two-photon calcium imaging, we determined that the striatal fiber photometry does not reflect spiking-related changes in calcium and instead primarily reflects nonsomatic changes in calcium.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-04-04T00:04:00.103Z","creation":"2025-04-04T00:04:00.103Z"},"accession":"S-EPMC10152879","cross_references":{"pubmed":["36042311"],"doi":["10.1038/s41593-022-01152-z"]}}