{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hansen LN"],"funding":["National Institutes of Health","NIGMS NIH HHS"],"pagination":["168070"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10152882"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["435(10)"],"pubmed_abstract":["The architecture and folding of complex RNAs is governed by a limited set of highly recurrent structural motifs that form long-range tertiary interactions. One of these motifs is the T-loop, which was first identified in tRNA but is broadly distributed across biological RNAs. While the T-loop has been examined in detail in different biological contexts, the various receptors that it interacts with are not as well defined. In this study, we use a cell-based genetic screen in concert with bioinformatic analysis to examine three different, but related, T-loop receptor motifs found in the flavin mononucleotide (FMN) and cobalamin (Cbl) riboswitches. As a host for different T-loop receptors, we employed the env8 class-II Cbl riboswitch, an RNA that uses two T-loop motifs for both folding and su"],"journal":["Journal of molecular biology"],"pubmed_title":["Context-dependence of T-loop Mediated Long-range RNA Tertiary Interactions."],"pmcid":["PMC10152882"],"funding_grant_id":["R01 GM073850"],"pubmed_authors":["Kletzien OA","Urquijo M","Hansen LN","Schwanz LT","Batey RT"],"additional_accession":[]},"is_claimable":false,"name":"Context-dependence of T-loop Mediated Long-range RNA Tertiary Interactions.","description":"The architecture and folding of complex RNAs is governed by a limited set of highly recurrent structural motifs that form long-range tertiary interactions. One of these motifs is the T-loop, which was first identified in tRNA but is broadly distributed across biological RNAs. While the T-loop has been examined in detail in different biological contexts, the various receptors that it interacts with are not as well defined. In this study, we use a cell-based genetic screen in concert with bioinformatic analysis to examine three different, but related, T-loop receptor motifs found in the flavin mononucleotide (FMN) and cobalamin (Cbl) riboswitches. As a host for different T-loop receptors, we employed the env8 class-II Cbl riboswitch, an RNA that uses two T-loop motifs for both folding and su","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2026-06-01T18:48:23.335Z","creation":"2026-05-19T03:07:06.481Z"},"accession":"S-EPMC10152882","cross_references":{"pubmed":["37003469"],"doi":["10.1016/j.jmb.2023.168070"]}}