biostudies-literatureBelhart KConsejo Nacional de Investigaciones Científicas y TécnicasInternational Union of Biochemestry and Molecular BiologyLSU Health ShreveportCenter of Excellence for Arthritis and RheumatologyUniversidad Nacional de La PlataUniversidad Nacional de La Plata,ArgentinaAgencia Nacional de Promoción Científica y TecnológicaLouisiana Board of RegentsNational Institutes of HealthNIH HHS7157https://www.ebi.ac.uk/biostudies/studies/S-EPMC10154355biostudies-literatureUnknown13(1)Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in different animals, including mice, making B. bronchiseptica the gold-standard model to investigate host-pathogen interaction at the molecular level. B. bronchiseptica utilizes many different mechanisms to precisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesized by diguanylate cyclases and degraded by phosphodiesterases that regulates the expression of multiple virulence factors including biofilm formation. As in other bacteria, we have previously shown that c-di-GMP regulates motility and biofilm formation in B. bronchiseptica. This work describes the diguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase that promotScientific reportsBordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response.PMC10154355NIH COBRE award 1P20-GM134974-0181PID-UNLP-X819Research CareerIUBMB Wood-Whelan Research FellowshipLEQSF(2022-25)-RD-A-33Research FellowshipPICT-2019-00680Intramural Research Council Seed package supportintramural awardSisti FGestal MCFernandez JBelhart KfalseBordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response.Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in different animals, including mice, making B. bronchiseptica the gold-standard model to investigate host-pathogen interaction at the molecular level. B. bronchiseptica utilizes many different mechanisms to precisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesized by diguanylate cyclases and degraded by phosphodiesterases that regulates the expression of multiple virulence factors including biofilm formation. As in other bacteria, we have previously shown that c-di-GMP regulates motility and biofilm formation in B. bronchiseptica. This work describes the diguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase that promot2023-01-01T00:00:00Z2023 May2025-04-05T00:11:49.561Z2025-04-05T00:11:49.561ZS-EPMC101543553713095810.1038/s41598-023-34106-x