{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["82"],"submitter":["Cleveland MH"],"funding":["National Institute of Standards and Technology"],"pubmed_abstract":["In response to the COVID-19 pandemic, the National Institute of Standards and Technology released a synthetic RNA material for SARS-CoV-2 in June 2020. The goal was to rapidly produce a material to support molecular diagnostic testing applications. This material, referred to as Research Grade Test Material 10169, was shipped free of charge to laboratories across the globe to provide a non-hazardous material for assay development and assay calibration. The material consisted of two unique regions of the SARS-CoV-2 genome approximately 4 kb nucleotides in length. The concentration of each synthetic fragment was measured using RT-dPCR methods and confirmed to be compatible with RT-qPCR methods. In this report, the preparation, stability, and limitations of this material are described."],"journal":["Biologicals : journal of the International Association of Biological Standardization"],"pagination":["101680"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10154543"],"repository":["biostudies-literature"],"pubmed_title":["Rapid production and free distribution of a synthetic RNA material to support SARS-CoV-2 molecular diagnostic testing."],"pmcid":["PMC10154543"],"pubmed_authors":["Valiant WG","Vallone PM","Romsos EL","Servetas SL","Steffen CR","Olson ND","Cleveland MH"],"additional_accession":[]},"is_claimable":false,"name":"Rapid production and free distribution of a synthetic RNA material to support SARS-CoV-2 molecular diagnostic testing.","description":"In response to the COVID-19 pandemic, the National Institute of Standards and Technology released a synthetic RNA material for SARS-CoV-2 in June 2020. The goal was to rapidly produce a material to support molecular diagnostic testing applications. This material, referred to as Research Grade Test Material 10169, was shipped free of charge to laboratories across the globe to provide a non-hazardous material for assay development and assay calibration. The material consisted of two unique regions of the SARS-CoV-2 genome approximately 4 kb nucleotides in length. The concentration of each synthetic fragment was measured using RT-dPCR methods and confirmed to be compatible with RT-qPCR methods. In this report, the preparation, stability, and limitations of this material are described.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2025-04-21T16:39:55.652Z","creation":"2025-04-05T10:31:58.221Z"},"accession":"S-EPMC10154543","cross_references":{"pubmed":["37178559"],"doi":["10.1016/j.biologicals.2023.101680"]}}