biostudies-literatureUnknown54(12)Liu WTurboID, a proximity labelling method based on mutant biotin ligase, is an efficient new technique for recognizing protein-protein interactions and has been successfully applied to living cells. Sialic acid is typically the terminal monosaccharide attached to many glycoproteins and plays many important roles in many biological processes. However, the lack of enrichment methods for terminal sialic acid glycosylation <i>in vivo</i> hinders the identification and analysis of this glycosylation. Here, we introduce TurboID to identify terminal sialic acid glycosylation in living cells. <i>Sp</i>CBM, the carbohydrate-binding domain of sialidase from <i>Streptococcus pneumoniae</i>, is fused with TurboID and overexpressed in HeLa cells. After streptavidin-based purification and detection by mass Acta biochimica et biophysica Sinica1841-1853https://www.ebi.ac.uk/biostudies/studies/S-EPMC10157534biostudies-literatureEfficient TurboID-based proximity labelling method for identifying terminal sialic acid glycosylation in living cells.PMC10157534Yang XLi YLong YBao YZhu HDeng MSu YLiu WfalseEfficient TurboID-based proximity labelling method for identifying terminal sialic acid glycosylation in living cells.TurboID, a proximity labelling method based on mutant biotin ligase, is an efficient new technique for recognizing protein-protein interactions and has been successfully applied to living cells. Sialic acid is typically the terminal monosaccharide attached to many glycoproteins and plays many important roles in many biological processes. However, the lack of enrichment methods for terminal sialic acid glycosylation <i>in vivo</i> hinders the identification and analysis of this glycosylation. Here, we introduce TurboID to identify terminal sialic acid glycosylation in living cells. <i>Sp</i>CBM, the carbohydrate-binding domain of sialidase from <i>Streptococcus pneumoniae</i>, is fused with TurboID and overexpressed in HeLa cells. After streptavidin-based purification and detection by mass 2022-01-01T00:00:00Z2022 Dec2026-07-14T20:06:15.827Z2025-02-19T04:29:24.168ZS-EPMC101575343678969210.3724/abbs.2022184