biostudies-literatureUnknown14Ye CStem respiration (<i>R</i> _s) plays a vital role in ecosystem carbon cycling. However, the measured efflux on the stem surface (<i>E</i> _s) is not always <i>in situ R</i> _s but only part of it. A previously proposed mass balance framework (MBF) attempted to explore the multiple partitioning pathways of <i>R</i> _s, including sap-flow-transported and internal storage of <i>R</i> _s, in addition to <i>E</i> _s. This study proposed stem photosynthesis as an additional partitioning pathway to the MBF. Correspondingly, a double-chamber apparatus was designed and applied on newly sprouted Moso bamboo (<i>Phyllostachys edulis</i>) in leafless and leaved stages. <i>R</i> _s of newly sprouted bamboo were twice as high in the lFrontiers in plant science1154232https://www.ebi.ac.uk/biostudies/studies/S-EPMC10158728biostudies-literaturePartitioning of respired CO₂ in newly sprouted Moso bamboo culms.PMC10158728Zeng QFang DYe CDu HMei THu KZhou GHolscher DZhou YBerninger FShi YfalsePartitioning of respired CO₂ in newly sprouted Moso bamboo culms.Stem respiration (<i>R</i> _s) plays a vital role in ecosystem carbon cycling. However, the measured efflux on the stem surface (<i>E</i> _s) is not always <i>in situ R</i> _s but only part of it. A previously proposed mass balance framework (MBF) attempted to explore the multiple partitioning pathways of <i>R</i> _s, including sap-flow-transported and internal storage of <i>R</i> _s, in addition to <i>E</i> _s. This study proposed stem photosynthesis as an additional partitioning pathway to the MBF. Correspondingly, a double-chamber apparatus was designed and applied on newly sprouted Moso bamboo (<i>Phyllostachys edulis</i>) in leafless and leaved stages. <i>R</i> _s of newly sprouted bamboo were twice as high in the l2023-01-01T00:00:00Z20232025-04-26T14:37:02.144Z2025-04-06T14:36:44.044ZS-EPMC101587283715213210.3389/fpls.2023.1154232