biostudies-literatureUnknown27Kubi JADelayed bone defect repairs lead to severe health and socioeconomic impacts on patients. Hence, there are increasing demands for medical interventions to promote bone defect healing. Recombinant proteins such as BMP-2 have been recognized as one of the powerful osteogenic substances that promote mesenchymal stem cells (MSCs) to osteoblast differentiation and are widely applied clinically for bone defect repairs. However, recent reports show that BMP-2 treatment has been associated with clinical adverse side effects such as ectopic bone formation, osteolysis and stimulation of inflammation. Here, we have identified one new osteogenic protein, named 'HKUOT-S2' protein, from <i>Dioscorea opposita</i> Thunb<i>.</i> Using the bone defect model, we have shown that the HKUOT-S2 protein can acceleBioactive materials429-446https://www.ebi.ac.uk/biostudies/studies/S-EPMC10160600biostudies-literatureA new osteogenic protein isolated from <i>Dioscorea opposita</i> Thunb accelerates bone defect healing through the mTOR signaling axis.PMC10160600Qiao WBrah ASLee KFSze SCWYeung KWKubi JALee YLCheung KMCfalseA new osteogenic protein isolated from <i>Dioscorea opposita</i> Thunb accelerates bone defect healing through the mTOR signaling axis.Delayed bone defect repairs lead to severe health and socioeconomic impacts on patients. Hence, there are increasing demands for medical interventions to promote bone defect healing. Recombinant proteins such as BMP-2 have been recognized as one of the powerful osteogenic substances that promote mesenchymal stem cells (MSCs) to osteoblast differentiation and are widely applied clinically for bone defect repairs. However, recent reports show that BMP-2 treatment has been associated with clinical adverse side effects such as ectopic bone formation, osteolysis and stimulation of inflammation. Here, we have identified one new osteogenic protein, named 'HKUOT-S2' protein, from <i>Dioscorea opposita</i> Thunb<i>.</i> Using the bone defect model, we have shown that the HKUOT-S2 protein can accele2023-01-01T00:00:00Z2023 Sep2025-04-22T00:30:26.925Z2025-04-05T19:36:12.2ZS-EPMC101606003715271010.1016/j.bioactmat.2023.04.018