{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Juras JA"],"funding":["National Institute of Neurological Disorders and Stroke","National Institute of Diabetes and Digestive and Kidney Diseases","NIA NIH HHS","NIDDK NIH HHS","NIA","National Cancer Institute","NCI NIH HHS","NINDS NIH HHS","National Institutes of Health"],"pagination":["100455"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10163000"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["3(4)"],"pubmed_abstract":["Brain glucose metabolism is highly heterogeneous among brain regions and continues postmortem. In particular, we demonstrate exhaustion of glycogen and glucose and an increase in lactate production during conventional rapid brain resection and preservation by liquid nitrogen. In contrast, we show that these postmortem changes are not observed with simultaneous animal sacrifice and <i>in situ</i> fixation with focused, high-power microwave. We further employ microwave fixation to define brain glucose metabolism in the mouse model of streptozotocin-induced type 1 diabetes. Using both total pool and isotope tracing analyses, we identified global glucose hypometabolism in multiple brain regions, evidenced by reduced <sup>13</sup>C enrichment into glycogen, glycolysis, and the tricarboxylic aci"],"journal":["Cell reports methods"],"pubmed_title":["<i>In situ</i> microwave fixation provides an instantaneous snapshot of the brain metabolome."],"pmcid":["PMC10163000"],"funding_grant_id":["R01 CA266004","P30 CA177558","R01 AG066653","R01 NS092552","F99CA264165","F99 CA264165","R01 DK122811","R35 NS116824","T32 CA165990","T32CA165990","P01 NS097197","R25 CA221765"],"pubmed_authors":["Sun RC","Smith BN","Wang C","Buoncristiani MD","Coburn PT","Sanders WC","Sun LPY","Webb MB","Bolton KE","Hawkinson TR","Bruntz RC","Williams MI","Gentry MS","Young LEA","Markussen KH","Conroy LR","Juras JA"],"additional_accession":[]},"is_claimable":false,"name":"<i>In situ</i> microwave fixation provides an instantaneous snapshot of the brain metabolome.","description":"Brain glucose metabolism is highly heterogeneous among brain regions and continues postmortem. In particular, we demonstrate exhaustion of glycogen and glucose and an increase in lactate production during conventional rapid brain resection and preservation by liquid nitrogen. In contrast, we show that these postmortem changes are not observed with simultaneous animal sacrifice and <i>in situ</i> fixation with focused, high-power microwave. We further employ microwave fixation to define brain glucose metabolism in the mouse model of streptozotocin-induced type 1 diabetes. Using both total pool and isotope tracing analyses, we identified global glucose hypometabolism in multiple brain regions, evidenced by reduced <sup>13</sup>C enrichment into glycogen, glycolysis, and the tricarboxylic aci","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2026-05-28T11:59:12.554Z","creation":"2024-11-20T21:55:54.488Z"},"accession":"S-EPMC10163000","cross_references":{"pubmed":["37159672"],"doi":["10.1016/j.crmeth.2023.100455"]}}