biostudies-literatureGutierrez Guarnizo SANIGMS NIH HHS2605https://www.ebi.ac.uk/biostudies/studies/S-EPMC10163012biostudies-literatureUnknown14(1)Leishmania is a unicellular protozoan that has a limited transcriptional control and mostly uses post-transcriptional regulation of gene expression, although the molecular mechanisms of the process are still poorly understood. Treatments of leishmaniasis, pathologies associated with Leishmania infections, are limited due to drug resistance. Here, we report dramatic differences in mRNA translation in antimony drug-resistant and sensitive strains at the full translatome level. The major differences (2431 differentially translated transcripts) were demonstrated in the absence of the drug pressure supporting that complex preemptive adaptations are needed to efficiently compensate for the loss of biological fitness once they are exposed to the antimony. In contrast, drug-resistant parasites expNature communicationsTranslational reprogramming as a driver of antimony-drug resistance in Leishmania.PMC10163012R15 GM146171Muskus CETikhonova EBKaramyshev ALGutierrez Guarnizo SAKaramysheva ZNfalseTranslational reprogramming as a driver of antimony-drug resistance in Leishmania.Leishmania is a unicellular protozoan that has a limited transcriptional control and mostly uses post-transcriptional regulation of gene expression, although the molecular mechanisms of the process are still poorly understood. Treatments of leishmaniasis, pathologies associated with Leishmania infections, are limited due to drug resistance. Here, we report dramatic differences in mRNA translation in antimony drug-resistant and sensitive strains at the full translatome level. The major differences (2431 differentially translated transcripts) were demonstrated in the absence of the drug pressure supporting that complex preemptive adaptations are needed to efficiently compensate for the loss of biological fitness once they are exposed to the antimony. In contrast, drug-resistant parasites exp2023-01-01T00:00:00Z2023 May2026-05-28T18:32:58.088Z2025-04-19T02:43:36.826ZS-EPMC101630123714729110.1038/s41467-023-38221-1