biostudies-literatureUnknown9(1)Li SB7-H3 is an attractive target for immunotherapy because of its high expression across multiple solid tumors, including prostate cancer, and restricted expression in normal tissues. Among various types of tumor immunotherapy, chimeric antigen receptor T (CAR-T) cell therapy has shown remarkable success in hematological tumors. However, the potency of CAR-T cell therapy in solid tumors is still limited. Here, we examined the expression of B7-H3 in prostate cancer tissues and cells and developed a second-generation CAR that specifically targets B7-H3 and CD28 as costimulatory receptor to explore its tumoricidal potential against prostate cancer in vitro and in vivo. The high expression of B7-H3 was detected on both the surface of PC3, DU145 and LNCaP cells and prostate cancer tissues. B7-H3 CCell death discovery147https://www.ebi.ac.uk/biostudies/studies/S-EPMC10164129biostudies-literatureB7-H3 specific CAR-T cells exhibit potent activity against prostate cancer.PMC10164129Wang MMa PLi HZheng JLi SZhang MWang GWang HMao LWu HfalseB7-H3 specific CAR-T cells exhibit potent activity against prostate cancer.B7-H3 is an attractive target for immunotherapy because of its high expression across multiple solid tumors, including prostate cancer, and restricted expression in normal tissues. Among various types of tumor immunotherapy, chimeric antigen receptor T (CAR-T) cell therapy has shown remarkable success in hematological tumors. However, the potency of CAR-T cell therapy in solid tumors is still limited. Here, we examined the expression of B7-H3 in prostate cancer tissues and cells and developed a second-generation CAR that specifically targets B7-H3 and CD28 as costimulatory receptor to explore its tumoricidal potential against prostate cancer in vitro and in vivo. The high expression of B7-H3 was detected on both the surface of PC3, DU145 and LNCaP cells and prostate cancer tissues. B7-H3 C2023-01-01T00:00:00Z2023 May2025-04-22T00:32:55.3Z2025-04-05T19:35:00.68ZS-EPMC101641293714972110.1038/s41420-023-01453-7