{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tshilenge KT"],"funding":["NIA NIH HHS","NINDS NIH HHS","National Institutes of Health","NIH HHS"],"pagination":["100534"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10165459"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(5)"],"pubmed_abstract":["Huntington's disease (HD) is a neurodegenerative disease caused by a CAG repeat expansion in the Huntingtin (HTT) gene. The resulting polyglutamine (polyQ) tract alters the function of the HTT protein. Although HTT is expressed in different tissues, the medium-spiny projection neurons (MSNs) in the striatum are particularly vulnerable in HD. Thus, we sought to define the proteome of human HD patient-derived MSNs. We differentiated HD72-induced pluripotent stem cells and isogenic controls into MSNs and carried out quantitative proteomic analysis. Using data-dependent acquisitions with FAIMS for label-free quantification on the Orbitrap Lumos mass spectrometer, we identified 6323 proteins with at least two unique peptides. Of these, 901 proteins were altered significantly more in the HD72-MS"],"journal":["Molecular & cellular proteomics : MCP"],"pubmed_title":["Proteomic Analysis of Huntington's Disease Medium Spiny Neurons Identifies Alterations in Lipid Droplets."],"pmcid":["PMC10165459"],"funding_grant_id":["R01-NS100529","S10 OD016281","R01 NS100529","1S10 OD016281","S10 OD028654","K99 AG065484"],"pubmed_authors":["Loureiro A","Battistoni E","Hetz C","Rose J","Tshilenge KT","Milani M","Naphade S","Wehrfritz C","Basisty N","Schilling B","Bons J","Lopez-Ramirez A","Gerencser AA","Song S","Aguirre CG","Mooney SD","Ellerby LM","Holtz A"],"additional_accession":[]},"is_claimable":false,"name":"Proteomic Analysis of Huntington's Disease Medium Spiny Neurons Identifies Alterations in Lipid Droplets.","description":"Huntington's disease (HD) is a neurodegenerative disease caused by a CAG repeat expansion in the Huntingtin (HTT) gene. The resulting polyglutamine (polyQ) tract alters the function of the HTT protein. Although HTT is expressed in different tissues, the medium-spiny projection neurons (MSNs) in the striatum are particularly vulnerable in HD. Thus, we sought to define the proteome of human HD patient-derived MSNs. We differentiated HD72-induced pluripotent stem cells and isogenic controls into MSNs and carried out quantitative proteomic analysis. Using data-dependent acquisitions with FAIMS for label-free quantification on the Orbitrap Lumos mass spectrometer, we identified 6323 proteins with at least two unique peptides. Of these, 901 proteins were altered significantly more in the HD72-MS","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2026-07-14T20:07:38.747Z","creation":"2025-04-06T14:37:29.319Z"},"accession":"S-EPMC10165459","cross_references":{"pubmed":["36958627"],"doi":["10.1016/j.mcpro.2023.100534"]}}