biostudies-literatureUnknown13Da F<i>Vibrio alginolyticus</i> is the common pathogen affecting various species of marine organisms. It has been demonstrated that <i>fliR</i> is a necessary virulence factor to adhere and infect their hosts for pathogenic bacteria. Frequent disease outbreaks in aquaculture have highlighted the necessity of developing effective vaccines. In the present study, in order to investigate the function of <i>fliR</i> in <i>V.alginolyticus</i>, the <i>fliR</i> deletion mutant Δ<i>fliR</i> was constructed and its biological properties were evaluated, additionally, the differences in gene expression levels between wild-type and Δ<i>fliR</i> were analyzed by transcriptomics. Finally, Δ<i>fliR</i> was used as a live attenuated vaccine to immunize grouper via the intraperitoneal route to evaluate its protFrontiers in cellular and infection microbiology1162299https://www.ebi.ac.uk/biostudies/studies/S-EPMC10166871biostudies-literatureCharacterization of <i>fliR</i>-deletion mutant Δ<i>fliR</i> from <i>Vibrio alginolyticus</i> and the evaluation as a live attenuated vaccine.PMC10166871Da FCai SLin GWan XJian JfalseCharacterization of <i>fliR</i>-deletion mutant Δ<i>fliR</i> from <i>Vibrio alginolyticus</i> and the evaluation as a live attenuated vaccine.<i>Vibrio alginolyticus</i> is the common pathogen affecting various species of marine organisms. It has been demonstrated that <i>fliR</i> is a necessary virulence factor to adhere and infect their hosts for pathogenic bacteria. Frequent disease outbreaks in aquaculture have highlighted the necessity of developing effective vaccines. In the present study, in order to investigate the function of <i>fliR</i> in <i>V.alginolyticus</i>, the <i>fliR</i> deletion mutant Δ<i>fliR</i> was constructed and its biological properties were evaluated, additionally, the differences in gene expression levels between wild-type and Δ<i>fliR</i> were analyzed by transcriptomics. Finally, Δ<i>fliR</i> was used as a live attenuated vaccine to immunize grouper via the intraperitoneal route to evaluate its prot2023-01-01T00:00:00Z20232025-04-04T11:50:08.403Z2025-04-04T11:50:08.403ZS-EPMC101668713718043710.3389/fcimb.2023.1162299