biostudies-literatureUnknown61(4)Lo MLBasal stem rot incidence caused by a white-rot fungus, Ganoderma boninense, is the major disease of oil palm in Southeast Asia. The rate of disease transmission and host damage are affected by variations in pathogen aggressiveness. Several other studies have used the disease severity index (DSI) to determine G. boninense aggressiveness levels while verifying disease using a culture-based method, which might not provide accurate results or be feasible in all cases. To differentiate G. boninense aggressiveness, we employed the DSI and vegetative growth measurement of infected oil palm seedlings. Disease confirmation was performed through scanning electron microscopy and molecular identification of fungal DNA from both infected tissue and fungi isolated from Ganoderma selective medium. Two-moJournal of microbiology (Seoul, Korea)449-459https://www.ebi.ac.uk/biostudies/studies/S-EPMC10167175biostudies-literatureComparison of Ganoderma boninense Isolate's Aggressiveness Using Infected Oil Palm Seedlings.PMC10167175Midot FChin MYMelling LLau SYLTung HJJee MSThanh TAVLo MLWong WCfalseComparison of Ganoderma boninense Isolate's Aggressiveness Using Infected Oil Palm Seedlings.Basal stem rot incidence caused by a white-rot fungus, Ganoderma boninense, is the major disease of oil palm in Southeast Asia. The rate of disease transmission and host damage are affected by variations in pathogen aggressiveness. Several other studies have used the disease severity index (DSI) to determine G. boninense aggressiveness levels while verifying disease using a culture-based method, which might not provide accurate results or be feasible in all cases. To differentiate G. boninense aggressiveness, we employed the DSI and vegetative growth measurement of infected oil palm seedlings. Disease confirmation was performed through scanning electron microscopy and molecular identification of fungal DNA from both infected tissue and fungi isolated from Ganoderma selective medium. Two-mo2023-01-01T00:00:00Z2023 Apr2026-05-28T08:25:24.132Z2025-04-05T21:11:11.993ZS-EPMC101671753709758710.1007/s12275-023-00040-w