biostudies-literatureDyal JCCR NIH HHSFrederick National Laboratory for Cancer ResearchCenters for Disease Control and PreventionCDC FoundationFIC NIH HHSNIMHD NIH HHSNCI NIH HHSNIHe849-e856https://www.ebi.ac.uk/biostudies/studies/S-EPMC10169428biostudies-literatureUnknown76(3)<h4>Background</h4>Long-term persistence of Ebola virus (EBOV) in immunologically privileged sites has been implicated in recent outbreaks of Ebola virus disease (EVD) in Guinea and the Democratic Republic of Congo. This study was designed to understand how the acute course of EVD, convalescence, and host immune and genetic factors may play a role in prolonged viral persistence in semen.<h4>Methods</h4>A cohort of 131 male EVD survivors in Liberia were enrolled in a case-case study. "Early clearers" were defined as those with 2 consecutive negative EBOV semen test results by real-time reverse-transcription polymerase chain reaction (rRT-PCR) ≥2 weeks apart within 1 year after discharge from the Ebola treatment unit or acute EVD. "Late clearers" had detectable EBOV RNA by rRT-PCR >1 year afClinical infectious diseases : an official publication of the Infectious Diseases Society of AmericaRisk Factors for Ebola Virus Persistence in Semen of Survivors in Liberia.PMC10169428HHSN261200800001EU2R TW011281HHSN261200800001CU54 MD007586Chiriboga DEHinrichs SHBradbury RSFankhauser JNichol STSu KAhmed RKiawu ATony HDGiah GJawara MMorales-Betoulle MESecor WEMassaquoi MOrone RMartin MPPark YHKromah MLFaikai MVonhm BTFlowers BNyenswah TRollin PECarrington MNDyal JFreeman EWilliams DESoka MJChoi MJRogier EKollie JZBowden KECannon DLAguinaga MDPPurpura LJGlaybo UHaberling DLPhillips CErvin EDKofman AGraziano JKamara KLinderman SLDesamu-Thorpe RKamara SKlena JDMushi RPlucinski MBrown SfalseRisk Factors for Ebola Virus Persistence in Semen of Survivors in Liberia.<h4>Background</h4>Long-term persistence of Ebola virus (EBOV) in immunologically privileged sites has been implicated in recent outbreaks of Ebola virus disease (EVD) in Guinea and the Democratic Republic of Congo. This study was designed to understand how the acute course of EVD, convalescence, and host immune and genetic factors may play a role in prolonged viral persistence in semen.<h4>Methods</h4>A cohort of 131 male EVD survivors in Liberia were enrolled in a case-case study. "Early clearers" were defined as those with 2 consecutive negative EBOV semen test results by real-time reverse-transcription polymerase chain reaction (rRT-PCR) ≥2 weeks apart within 1 year after discharge from the Ebola treatment unit or acute EVD. "Late clearers" had detectable EBOV RNA by rRT-PCR >1 year af2023-01-01T00:00:00Z2023 Feb2025-04-05T10:54:30.657Z2025-04-05T10:54:30.657ZS-EPMC101694283563987510.1093/cid/ciac424