biostudies-literatureUnknown190(6)Jimenez MSUniversidad de ZaragozaExtractions methods based on ultrapure water, tetramethylammonium hydroxide (TMAH), and tetrasodium pyrophosphate (TSPP) were applied to faeces collected from two in vivo experiments of pigs and chickens fed with a silver-based nanomaterial to study the fate and speciation of silver. For TMAH extraction, cysteine and CaCl₂ were used to evaluate their stabilization effect on the silver forms. The analytical techniques single-particle inductively coupled plasma mass spectrometry (SP-ICP-MS), hydrodynamic chromatography hyphenated to ICP-MS (HDC-ICP-MS) and asymmetric flow field flow fractionation coupled to ICP-MS (AF4-ICP-MS) were applied to the simultaneous detection of particulate and dissolved silver. Results have shown that water extraction was a suitable option to assess theMikrochimica acta204https://www.ebi.ac.uk/biostudies/studies/S-EPMC10169895biostudies-literatureComparative study of extraction methods of silver species from faeces of animals fed with silver-based nanomaterials.PMC10169895Jimenez MSBakir MBolea ELaborda FBen-Jeddou KPerez-Arantegui JfalseComparative study of extraction methods of silver species from faeces of animals fed with silver-based nanomaterials.Extractions methods based on ultrapure water, tetramethylammonium hydroxide (TMAH), and tetrasodium pyrophosphate (TSPP) were applied to faeces collected from two in vivo experiments of pigs and chickens fed with a silver-based nanomaterial to study the fate and speciation of silver. For TMAH extraction, cysteine and CaCl₂ were used to evaluate their stabilization effect on the silver forms. The analytical techniques single-particle inductively coupled plasma mass spectrometry (SP-ICP-MS), hydrodynamic chromatography hyphenated to ICP-MS (HDC-ICP-MS) and asymmetric flow field flow fractionation coupled to ICP-MS (AF4-ICP-MS) were applied to the simultaneous detection of particulate and dissolved silver. Results have shown that water extraction was a suitable option to assess the2023-01-01T00:00:00Z2023 May2025-04-27T02:22:08.807Z2025-02-19T02:56:41.127ZS-EPMC101698953716077410.1007/s00604-023-05777-0