biostudies-literatureLiu FNatural Science Foundation of Hubei ProvinceFundamental Research Funds for the Central UniversitiesNational Natural Science Foundation of China118https://www.ebi.ac.uk/biostudies/studies/S-EPMC10170830biostudies-literatureUnknown42(1)<h4>Background</h4>The failure of novel therapies effective in preclinical animal models largely reflects the fact that current models do not really mimic the pathological/therapeutic features of glioblastoma (GBM), in which the most effective temozolomide chemoradiotherapy (RT/TMZ) regimen can only slightly extend survival. How to improve RT/TMZ efficacy remains a major challenge in clinic.<h4>Methods</h4>Syngeneic G422^TN-GBM model mice were subject to RT/TMZ, surgery, piperlongumine (PL), αPD1, glutathione. Metabolomics or transcriptomics data from G422^TN-GBM and human GBM were used for gene enrichment analysis and estimation of ROS generation/scavenging balance, oxidative stress damage, inflammation and immune cell infiltration. Overall survival, bioluminescent imaJournal of experimental & clinical cancer research : CRPiperlongumine conquers temozolomide chemoradiotherapy resistance to achieve immune cure in refractory glioblastoma via boosting oxidative stress-inflamation-CD8⁺-T cell immunity.PMC10170830819723622022CFB428821731972021GCRC0738187385481974468Zhang TTYin SLZhou QPeng JYJiang HFWu JXChen XQCao XPan FMiao CXu SJHe XGao PPLiu FXu XHfalsePiperlongumine conquers temozolomide chemoradiotherapy resistance to achieve immune cure in refractory glioblastoma via boosting oxidative stress-inflamation-CD8⁺-T cell immunity.<h4>Background</h4>The failure of novel therapies effective in preclinical animal models largely reflects the fact that current models do not really mimic the pathological/therapeutic features of glioblastoma (GBM), in which the most effective temozolomide chemoradiotherapy (RT/TMZ) regimen can only slightly extend survival. How to improve RT/TMZ efficacy remains a major challenge in clinic.<h4>Methods</h4>Syngeneic G422^TN-GBM model mice were subject to RT/TMZ, surgery, piperlongumine (PL), αPD1, glutathione. Metabolomics or transcriptomics data from G422^TN-GBM and human GBM were used for gene enrichment analysis and estimation of ROS generation/scavenging balance, oxidative stress damage, inflammation and immune cell infiltration. Overall survival, bioluminescent ima2023-01-01T00:00:00Z2023 May2025-04-22T14:42:09.057Z2025-04-06T01:06:17.496ZS-EPMC101708303716145010.1186/s13046-023-02686-1