{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bochicchio D"],"funding":["GlaxoSmithKline","Alzheimer Research UK","FP7 Health"],"pagination":["596-605"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10172255"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(3)"],"pubmed_abstract":["Long-term memory requires stable protein synthesis and is altered in Alzheimer's disease (AD). This study aimed to implement a method to measure the cerebral protein synthesis rate (PSR) with [11C]leucine PET in vivo in rats and evaluate potential PSR alterations longitudinally (6, 12 and 18 months old) in the TgF344-AD rat model of AD. Wistar, wild-type (WT) and TgF344-AD rats (TG) were scanned for 60 min with [11C]leucine. Arterial blood activity was monitored online and with discrete whole blood and plasma samples by γ-counting in Wistar rats, WT (n = 4) and TG (n = 5). Unlabelled amino acids were measured in plasma. The sensitivity of [11C]leucine PET to measure alterations in PSR was assessed in Wistar rats by injection of PSR inhibitor anisomycin before PET acquisition. Anisomycin ad"],"journal":["Molecular imaging and biology"],"pubmed_title":["Measurement of Protein Synthesis Rate in Rat by [11C]Leucine PET Imaging: Application to the TgF344-AD Model of Alzheimer's Disease."],"pmcid":["PMC10172255"],"funding_grant_id":["HEALTH-F2-2011-278850"],"pubmed_authors":["Parker CA","Bochicchio D","Boutin H","Herholz K","Hinz R","Christie L","Lawrence CB"],"additional_accession":[]},"is_claimable":false,"name":"Measurement of Protein Synthesis Rate in Rat by [11C]Leucine PET Imaging: Application to the TgF344-AD Model of Alzheimer's Disease.","description":"Long-term memory requires stable protein synthesis and is altered in Alzheimer's disease (AD). This study aimed to implement a method to measure the cerebral protein synthesis rate (PSR) with [11C]leucine PET in vivo in rats and evaluate potential PSR alterations longitudinally (6, 12 and 18 months old) in the TgF344-AD rat model of AD. Wistar, wild-type (WT) and TgF344-AD rats (TG) were scanned for 60 min with [11C]leucine. Arterial blood activity was monitored online and with discrete whole blood and plasma samples by γ-counting in Wistar rats, WT (n = 4) and TG (n = 5). Unlabelled amino acids were measured in plasma. The sensitivity of [11C]leucine PET to measure alterations in PSR was assessed in Wistar rats by injection of PSR inhibitor anisomycin before PET acquisition. Anisomycin ad","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2025-04-22T08:51:33.113Z","creation":"2025-04-05T22:43:22.905Z"},"accession":"S-EPMC10172255","cross_references":{"pubmed":["36538180"],"doi":["10.1007/s11307-022-01796-0"]}}