biostudies-literatureOsman AEGNIA NIH HHSNCI NIH HHS1910-1914https://www.ebi.ac.uk/biostudies/studies/S-EPMC10172868biostudies-literatureUnknown7(9)Clonal hematopoiesis (CH) represents clonal expansion of mutated hematopoietic stem cells detectable in the peripheral blood or bone marrow through next generation sequencing. The current prevailing model posits that CH mutations detected in the peripheral blood mirror bone marrow mutations with clones widely disseminated across hematopoietic compartments. We sought to test the hypothesis that all clones are disseminated throughout hematopoietic tissues by comparing CH in hip vs peripheral blood specimens collected at the time of hip replacement surgery. Here, we show that patients with osteoarthritis have a high prevalence of CH, which involve genes encoding epigenetic modifiers and DNA damage repair pathway proteins. Importantly, we illustrate that CH, including clones with variant allelBlood advancesPaired bone marrow and peripheral blood samples demonstrate lack of widespread dissemination of some CH clones.PMC10172868R21 AG066552R01 CA248747Kim AMencia-Trinchant NSinha EDiNardi NOsman AEGJabri BDesai PHassane DCWallace SSGodley LASaygin CLuu HMoma LPozsgai MSboner AGuzman MLHousman GChandra PJohnson CSangani KfalsePaired bone marrow and peripheral blood samples demonstrate lack of widespread dissemination of some CH clones.Clonal hematopoiesis (CH) represents clonal expansion of mutated hematopoietic stem cells detectable in the peripheral blood or bone marrow through next generation sequencing. The current prevailing model posits that CH mutations detected in the peripheral blood mirror bone marrow mutations with clones widely disseminated across hematopoietic compartments. We sought to test the hypothesis that all clones are disseminated throughout hematopoietic tissues by comparing CH in hip vs peripheral blood specimens collected at the time of hip replacement surgery. Here, we show that patients with osteoarthritis have a high prevalence of CH, which involve genes encoding epigenetic modifiers and DNA damage repair pathway proteins. Importantly, we illustrate that CH, including clones with variant allel2023-01-01T00:00:00Z2023 May2025-04-05T10:55:06.699Z2025-04-05T10:55:06.699ZS-EPMC101728683645364110.1182/bloodadvances.2022008521