{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Luo D"],"funding":["Chinese Academy of Sciences"],"pagination":["445-453"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10173069"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["32"],"pubmed_abstract":["High purity is essential in mRNA-based therapeutic applications. A major contaminant of <i>in vitro</i>-transcribed (IVT) mRNA manufacturing is double-stranded RNA (dsRNA), which can induce severe anti-viral immune responses. Detection methods, such as agarose gel electrophoresis, ELISA, and dot-blot assay, are used to detect the existence of dsRNA in IVT mRNA products. However, these methods are either not sensitive enough or time-consuming. To overcome these challenges, we develop a rapid, sensitive, and easy-to-implement colloidal gold nanoparticle-based lateral flow strip assay (LFSA) with sandwich format for the detection of dsRNA from IVT process. dsRNA contaminant can be determined visually on the test strip or quantitatively with a portable optical detector. This method allows for "],"journal":["Molecular therapy. Nucleic acids"],"pubmed_title":["Lateral flow immunoassay for rapid and sensitive detection of dsRNA contaminants in <i>in vitro</i>-transcribed mRNA products."],"pmcid":["PMC10173069"],"funding_grant_id":["YSBR010"],"pubmed_authors":["Shao F","Wu Z","Cestellos-Blanco S","Zhang J","Wang S","Wang D","Cao Y","Xu D","Luo D"],"additional_accession":[]},"is_claimable":false,"name":"Lateral flow immunoassay for rapid and sensitive detection of dsRNA contaminants in <i>in vitro</i>-transcribed mRNA products.","description":"High purity is essential in mRNA-based therapeutic applications. A major contaminant of <i>in vitro</i>-transcribed (IVT) mRNA manufacturing is double-stranded RNA (dsRNA), which can induce severe anti-viral immune responses. Detection methods, such as agarose gel electrophoresis, ELISA, and dot-blot assay, are used to detect the existence of dsRNA in IVT mRNA products. However, these methods are either not sensitive enough or time-consuming. To overcome these challenges, we develop a rapid, sensitive, and easy-to-implement colloidal gold nanoparticle-based lateral flow strip assay (LFSA) with sandwich format for the detection of dsRNA from IVT process. dsRNA contaminant can be determined visually on the test strip or quantitatively with a portable optical detector. This method allows for ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2025-04-05T09:12:02.147Z","creation":"2025-04-05T09:12:02.147Z"},"accession":"S-EPMC10173069","cross_references":{"pubmed":["37181450"],"doi":["10.1016/j.omtn.2023.04.005"]}}