biostudies-literatureIlyas MPrincess Nourah Bint Abdulrahman University16327-16344https://www.ebi.ac.uk/biostudies/studies/S-EPMC10173433biostudies-literatureUnknown8(18)Germin (GER) and germin-like proteins (GLPs) play an important role in various plant processes. <i>Zea mays</i> contains 26 germin-like protein genes (<i>ZmGLPs</i>) located on chromosomes 2, 4, and 10; most of which are functionally unexplored. The present study aimed to characterize all <i>ZmGLPs</i> using the latest computational tools. All of them were studied at a physicochemical, subcellular, structural, and functional level, and their expression was predicted in plant development, against biotic and abiotic stresses using various <i>in silico</i> approaches. Overall, ZmGLPs showed greater similarity in their physicochemical properties, domain architecture, and structure, mostly localized in the cytoplasmic or extracellular regions. Phylogenetically, they have a narrow genetic backgrACS omegaMolecular Characterization of Germin-like Protein Genes in <i>Zea mays</i> (<i>ZmGLPs</i>) Using Various <i>In Silico</i> Approaches.PMC10173433PNURSP2023R155Mahmood TAli IUllah SBokhari SHAAli BNasser Binjawhar DEldin SMIqbal RIlyas MfalseMolecular Characterization of Germin-like Protein Genes in <i>Zea mays</i> (<i>ZmGLPs</i>) Using Various <i>In Silico</i> Approaches.Germin (GER) and germin-like proteins (GLPs) play an important role in various plant processes. <i>Zea mays</i> contains 26 germin-like protein genes (<i>ZmGLPs</i>) located on chromosomes 2, 4, and 10; most of which are functionally unexplored. The present study aimed to characterize all <i>ZmGLPs</i> using the latest computational tools. All of them were studied at a physicochemical, subcellular, structural, and functional level, and their expression was predicted in plant development, against biotic and abiotic stresses using various <i>in silico</i> approaches. Overall, ZmGLPs showed greater similarity in their physicochemical properties, domain architecture, and structure, mostly localized in the cytoplasmic or extracellular regions. Phylogenetically, they have a narrow genetic backgr2023-01-01T00:00:00Z2023 May2026-05-29T10:39:27.994Z2024-10-16T17:12:13.946ZS-EPMC101734333717962010.1021/acsomega.3c01104