biostudies-literatureZhuang Jthe Guangdong Medical Research Fundthe Open project of the Key Laboratory of Male Reproduction and Genetics of the National Health CommissionGuangdong Basic and Applied Basic Research FoundationBasic and Applied Basic Research Foundation of Guangdong ProvinceNational Natural Science Foundation of ChinaGuangdong Provincial Reproductive Science Institute Innovation Team grants21https://www.ebi.ac.uk/biostudies/studies/S-EPMC10173474biostudies-literatureUnknown20(1)<h4>Background</h4>Morphological and functional alterations in aging reproductive organs result in decreased male fertility. The epididymis functions as the transition region for post-testicular sperm maturation. And we have previously demonstrated that the epididymal initial segment (IS), a region of the reproductive tract essential for sperm maturation and capacitation, undergoes considerable histological changes and chronic immune activation in mice during aging. However, the local aging-associated cellular and molecular changes in the aged epididymal IS are poorly understood.<h4>Results</h4>We conducted single-cell RNA sequencing analysis on the epididymal IS of young (3-month-old) and old (21-month-old) mice. In total, 10,027 cells from the epididymal IS tissues of young and old mice Immunity & ageing : I & ASingle-cell RNA sequencing reveals the local cell landscape in mouse epididymal initial segment during aging.PMC10173474KF202001Nos. 2020A1515111160, 2021A1515110986 and 2020A1515110735No.C-03Nos. B2021333 and B2021453Nos.82101819 and 82101664Liu HLi XYao JLiu JHu YXie YQin WSun XZhuang JNie HTu XfalseSingle-cell RNA sequencing reveals the local cell landscape in mouse epididymal initial segment during aging.<h4>Background</h4>Morphological and functional alterations in aging reproductive organs result in decreased male fertility. The epididymis functions as the transition region for post-testicular sperm maturation. And we have previously demonstrated that the epididymal initial segment (IS), a region of the reproductive tract essential for sperm maturation and capacitation, undergoes considerable histological changes and chronic immune activation in mice during aging. However, the local aging-associated cellular and molecular changes in the aged epididymal IS are poorly understood.<h4>Results</h4>We conducted single-cell RNA sequencing analysis on the epididymal IS of young (3-month-old) and old (21-month-old) mice. In total, 10,027 cells from the epididymal IS tissues of young and old mice 2023-01-01T00:00:00Z2023 May2025-04-22T14:40:07.587Z2025-04-06T01:07:58.223ZS-EPMC101734743717032510.1186/s12979-023-00345-9