{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Boudreau-Pinsonneault C"],"funding":["Fighting Blindness Canada","FRQ | Fonds de Recherche du Québec - Santé","Krembil Foundation","Gouvernement du Canada | Canadian Institutes of Health Research","CIHR","FRQS Vision Health Research Network"],"pagination":["e2122168120"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10175841"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["120(19)"],"pubmed_abstract":["Temporal identity factors are sufficient to reprogram developmental competence of neural progenitors and shift cell fate output, but whether they can also reprogram the identity of terminally differentiated cells is unknown. To address this question, we designed a conditional gene expression system that allows rapid screening of potential reprogramming factors in mouse retinal glial cells combined with genetic lineage tracing. Using this assay, we found that coexpression of the early temporal identity transcription factors Ikzf1 and Ikzf4 is sufficient to directly convert Müller glial (MG) cells into cells that translocate to the outer nuclear layer (ONL), where photoreceptor cells normally reside. We name these \"induced ONL (iONL)\" cells. Using genetic lineage tracing, histological, immun"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Direct neuronal reprogramming by temporal identity factors."],"pmcid":["PMC10175841"],"funding_grant_id":["NA","PJT-166032","PJT-166074","FDN-159936"],"pubmed_authors":["Boudreau-Pinsonneault C","Cayouette M","Lourenco Fernandes JA","Fries M","David LA","Mattar P","Javed A"],"additional_accession":[]},"is_claimable":false,"name":"Direct neuronal reprogramming by temporal identity factors.","description":"Temporal identity factors are sufficient to reprogram developmental competence of neural progenitors and shift cell fate output, but whether they can also reprogram the identity of terminally differentiated cells is unknown. To address this question, we designed a conditional gene expression system that allows rapid screening of potential reprogramming factors in mouse retinal glial cells combined with genetic lineage tracing. Using this assay, we found that coexpression of the early temporal identity transcription factors Ikzf1 and Ikzf4 is sufficient to directly convert Müller glial (MG) cells into cells that translocate to the outer nuclear layer (ONL), where photoreceptor cells normally reside. We name these \"induced ONL (iONL)\" cells. Using genetic lineage tracing, histological, immun","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2026-07-15T01:42:06.412Z","creation":"2025-04-05T21:08:27.828Z"},"accession":"S-EPMC10175841","cross_references":{"pubmed":["37126716"],"doi":["10.1073/pnas.2122168120"]}}