{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Dvorak J"],"funding":["European Regional Development Fund-Project \"UP CIISB \"","Czech Academy of Sciences","MEYS CR","Charles University Grant Agency","Medical Research Foundation of the Czech Republic","European Regional Development Fund-Project "UP CIISB "","National Recovery Plan"],"pagination":["20"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10176672"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(1)"],"pubmed_abstract":["

Background

Sepsis is a common worldwide health condition with high mortality. It is caused by a dysregulated immune response to the pathogen. Severe infections resulting in sepsis can be also determined by monitoring several bloodstream biomarkers, one of them being pro-hormone procalcitonin (PCT). PCT concentration in the bloodstream correlates well with sepsis and in severe cases increases up to a thousand times from the healthy physiological values in a short time. In this study, we developed a rapid technique for PCT detection by MALDI-TOF mass spectrometry, that uses in-situ enrichment directly on the specialized immuno MALDI chips that are utilized as MALDI plates. The method's ability to detect PCT was confirmed by comparing the results with LC-MS bottom-up workflow. The new"],"journal":["Clinical proteomics"],"pubmed_title":["The rapid detection of procalcitonin in septic serum using immunoaffinity MALDI chips."],"pmcid":["PMC10176672"],"funding_grant_id":["384021","RVO61388971","LX22NPO5103","LM2018127","CZ.02.1.01/0.0/0.0/18_046/0015974","NV19-05-00541"],"pubmed_authors":["Horak J","Dvorak J","Pompach P","Karvunidis T","Kralovcova M","Hrabak J","Volny M","Kraftova L","Radej J","Novak P","Matejovic M","Novakova J","Kalaninova Z","Studentova V"],"additional_accession":[]},"is_claimable":false,"name":"The rapid detection of procalcitonin in septic serum using immunoaffinity MALDI chips.","description":"

Background

Sepsis is a common worldwide health condition with high mortality. It is caused by a dysregulated immune response to the pathogen. Severe infections resulting in sepsis can be also determined by monitoring several bloodstream biomarkers, one of them being pro-hormone procalcitonin (PCT). PCT concentration in the bloodstream correlates well with sepsis and in severe cases increases up to a thousand times from the healthy physiological values in a short time. In this study, we developed a rapid technique for PCT detection by MALDI-TOF mass spectrometry, that uses in-situ enrichment directly on the specialized immuno MALDI chips that are utilized as MALDI plates. The method's ability to detect PCT was confirmed by comparing the results with LC-MS bottom-up workflow. The new","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2026-05-15T03:17:39.333Z","creation":"2025-02-19T02:23:05.727Z"},"accession":"S-EPMC10176672","cross_references":{"pubmed":["37170190"],"doi":["10.1186/s12014-023-09410-3"]}}