biostudies-literatureDvorak JEuropean Regional Development Fund-Project "UP CIISB "Czech Academy of SciencesMEYS CRCharles University Grant AgencyMedical Research Foundation of the Czech RepublicEuropean Regional Development Fund-Project &quot;UP CIISB &quot;National Recovery Plan20https://www.ebi.ac.uk/biostudies/studies/S-EPMC10176672biostudies-literatureUnknown20(1)<h4>Background</h4>Sepsis is a common worldwide health condition with high mortality. It is caused by a dysregulated immune response to the pathogen. Severe infections resulting in sepsis can be also determined by monitoring several bloodstream biomarkers, one of them being pro-hormone procalcitonin (PCT). PCT concentration in the bloodstream correlates well with sepsis and in severe cases increases up to a thousand times from the healthy physiological values in a short time. In this study, we developed a rapid technique for PCT detection by MALDI-TOF mass spectrometry, that uses in-situ enrichment directly on the specialized immuno MALDI chips that are utilized as MALDI plates. The method's ability to detect PCT was confirmed by comparing the results with LC-MS bottom-up workflow. The newClinical proteomicsThe rapid detection of procalcitonin in septic serum using immunoaffinity MALDI chips.PMC10176672384021RVO61388971LX22NPO5103LM2018127CZ.02.1.01/0.0/0.0/18_046/0015974NV19-05-00541Horak JDvorak JPompach PKarvunidis TKralovcova MHrabak JVolny MKraftova LRadej JNovak PMatejovic MNovakova JKalaninova ZStudentova VfalseThe rapid detection of procalcitonin in septic serum using immunoaffinity MALDI chips.<h4>Background</h4>Sepsis is a common worldwide health condition with high mortality. It is caused by a dysregulated immune response to the pathogen. Severe infections resulting in sepsis can be also determined by monitoring several bloodstream biomarkers, one of them being pro-hormone procalcitonin (PCT). PCT concentration in the bloodstream correlates well with sepsis and in severe cases increases up to a thousand times from the healthy physiological values in a short time. In this study, we developed a rapid technique for PCT detection by MALDI-TOF mass spectrometry, that uses in-situ enrichment directly on the specialized immuno MALDI chips that are utilized as MALDI plates. The method's ability to detect PCT was confirmed by comparing the results with LC-MS bottom-up workflow. The new2023-01-01T00:00:00Z2023 May2026-05-15T03:17:39.333Z2025-02-19T02:23:05.727ZS-EPMC101766723717019010.1186/s12014-023-09410-3