{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang X"],"funding":["Shanghai Science& Technology Innovation Action Program","Shanghai Public Health Clinical Center hospital-level scientific research project"],"pagination":["1530"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10177487"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(9)"],"pubmed_abstract":["Loop-mediated isothermal amplification (LAMP), as the rank one alternative to a polymerase chain reaction (PCR), has been widely applied in point-of-care testing (POCT) due to its rapid, simple, and cost-effective characteristics. However, it is difficult to achieve real-time monitoring and multiplex detection with the traditional LAMP method. In addition, these approaches that use turbidimetry, sequence-independent intercalating dyes, or pH-sensitive indicators to indirectly reflect amplification can result in false-positive results if non-specific amplification occurs. To fulfill the needs of specific target detection and one-pot multiplex detection, a variety of probe-based LAMP assays have been developed. This review focuses on the principles of these assays, summarizes their applicati"],"journal":["Diagnostics (Basel, Switzerland)"],"pubmed_title":["Evolution of the Probe-Based Loop-Mediated Isothermal Amplification (LAMP) Assays in Pathogen Detection."],"pmcid":["PMC10177487"],"funding_grant_id":["KY -GW-2023-32","21Y11900500"],"pubmed_authors":["Zhang X","Zeng Y","Zhang C","Zhao Y"],"additional_accession":[]},"is_claimable":false,"name":"Evolution of the Probe-Based Loop-Mediated Isothermal Amplification (LAMP) Assays in Pathogen Detection.","description":"Loop-mediated isothermal amplification (LAMP), as the rank one alternative to a polymerase chain reaction (PCR), has been widely applied in point-of-care testing (POCT) due to its rapid, simple, and cost-effective characteristics. However, it is difficult to achieve real-time monitoring and multiplex detection with the traditional LAMP method. In addition, these approaches that use turbidimetry, sequence-independent intercalating dyes, or pH-sensitive indicators to indirectly reflect amplification can result in false-positive results if non-specific amplification occurs. To fulfill the needs of specific target detection and one-pot multiplex detection, a variety of probe-based LAMP assays have been developed. This review focuses on the principles of these assays, summarizes their applicati","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2025-04-22T00:33:23.392Z","creation":"2025-04-05T19:35:12.246Z"},"accession":"S-EPMC10177487","cross_references":{"pubmed":["37174922"],"doi":["10.3390/diagnostics13091530"]}}