biostudies-literatureAbuja PMAustrian Federal Ministry for Digital and Economic AffairsAustrian Science Fund FWFAustrian Infrastructure Program 2016/2017National Foundation for Research and DevelopmentEuropean UnionChristian Doppler Research AssociationAustrian Research Promotion AgencyIntegrative Metabolism Research Center GrazAustrian Federal Ministry for Education, Science and ResearchBioTechMed GrazStyrian GovernmentCity of Graz8010https://www.ebi.ac.uk/biostudies/studies/S-EPMC10178321biostudies-literatureUnknown24(9)Molecular diagnostics in healthcare relies increasingly on genomic and transcriptomic methodologies and requires appropriate tissue specimens from which nucleic acids (NA) of sufficiently high quality can be obtained. Besides the duration of ischemia and fixation type, NA quality depends on a variety of other pre-analytical parameters, such as storage conditions and duration. It has been discussed that the improper dehydration of tissue during processing influences the quality of NAs and the shelf life of fixed tissue. Here, we report on establishing a method for determining the amount of residual water in fixed, paraffin-embedded tissue (fixed by neutral buffered formalin or a non-crosslinking fixative) and its correlation to the performance of NAs in quantitative real-time polymerase chaInternational journal of molecular sciencesResidual Humidity in Paraffin-Embedded Tissue Reduces Nucleic Acid Stability.PMC10178321n/aStyrian Government Zukunftsfonds, doc.fund programmeP28854, I3792, DOC-130, DK-MCDW1226733112864690, 870454flagship project824110BMBFW 10.470/0010-V/3c/2018Bourgeois BZatloukal KPabst DLoibner MUlz CKraemer RAbuja PMMadl TStumptner CFackelmann UKufferath IfalseResidual Humidity in Paraffin-Embedded Tissue Reduces Nucleic Acid Stability.Molecular diagnostics in healthcare relies increasingly on genomic and transcriptomic methodologies and requires appropriate tissue specimens from which nucleic acids (NA) of sufficiently high quality can be obtained. Besides the duration of ischemia and fixation type, NA quality depends on a variety of other pre-analytical parameters, such as storage conditions and duration. It has been discussed that the improper dehydration of tissue during processing influences the quality of NAs and the shelf life of fixed tissue. Here, we report on establishing a method for determining the amount of residual water in fixed, paraffin-embedded tissue (fixed by neutral buffered formalin or a non-crosslinking fixative) and its correlation to the performance of NAs in quantitative real-time polymerase cha2023-01-01T00:00:00Z2023 Apr2025-04-05T10:55:06.263Z2025-04-05T10:55:06.263ZS-EPMC101783213717571610.3390/ijms24098010