{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["24(9)"],"submitter":["Temeyer KB"],"pubmed_abstract":["A cDNA encoding a novel cholinesterase (ChE, EC 3.1.1.8) from the larvae of <i>Amblyomma americanum</i> (Linnaeus) was identified, sequenced, and expressed in <i>Sf21</i> insect cell culture using the baculoviral expression vector pBlueBac4.5/V5-His. The open reading frame (1746 nucleotides) of the cDNA encoded 581 amino acids beginning with the initiation codon. Identical cDNA sequences were amplified from the total RNA of adult tick synganglion and salivary gland, strongly suggesting expression in both tick synganglion and saliva. The recombinant enzyme (rAaChE1) was highly sensitive to eserine and BW284c51, relatively insensitive to tetraisopropyl pyrophosphoramide (iso-OMPA) and ethopropazine, and hydrolyzed butyrylthiocholine (BuTCh) 5.7 times as fast as acetylthiocholine (ATCh) at 12"],"journal":["International journal of molecular sciences"],"pagination":["7681"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10178864"],"repository":["biostudies-literature"],"pubmed_title":["Identification, Baculoviral Expression, and Biochemical Characterization of a Novel Cholinesterase of <i>Amblyomma americanum</i> (Acari: Ixodidae)."],"pmcid":["PMC10178864"],"pubmed_authors":["Schlechte KG","Gross AD","Temeyer KB","Lohmeyer KH"],"additional_accession":[]},"is_claimable":false,"name":"Identification, Baculoviral Expression, and Biochemical Characterization of a Novel Cholinesterase of <i>Amblyomma americanum</i> (Acari: Ixodidae).","description":"A cDNA encoding a novel cholinesterase (ChE, EC 3.1.1.8) from the larvae of <i>Amblyomma americanum</i> (Linnaeus) was identified, sequenced, and expressed in <i>Sf21</i> insect cell culture using the baculoviral expression vector pBlueBac4.5/V5-His. The open reading frame (1746 nucleotides) of the cDNA encoded 581 amino acids beginning with the initiation codon. Identical cDNA sequences were amplified from the total RNA of adult tick synganglion and salivary gland, strongly suggesting expression in both tick synganglion and saliva. The recombinant enzyme (rAaChE1) was highly sensitive to eserine and BW284c51, relatively insensitive to tetraisopropyl pyrophosphoramide (iso-OMPA) and ethopropazine, and hydrolyzed butyrylthiocholine (BuTCh) 5.7 times as fast as acetylthiocholine (ATCh) at 12","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2025-04-04T13:07:53.696Z","creation":"2025-04-04T13:07:53.696Z"},"accession":"S-EPMC10178864","cross_references":{"pubmed":["37175388"],"doi":["10.3390/ijms24097681"]}}