{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tse CM"],"funding":["Cystic Fibrosis Foundation grant","nih ro1","National Institutes of Health grants"],"pagination":["8273"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10179158"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(9)"],"pubmed_abstract":["Cholesterol-rich membrane domains, also called lipid rafts (LRs), are specialized membrane domains that provide a platform for intracellular signal transduction. Membrane proteins often cluster in LRs that further aggregate into larger platform-like structures that are enriched in ceramides and are called ceramide-rich platforms (CRPs). The role of CRPs in the regulation of intestinal epithelial functions remains unknown. Down-regulated in adenoma (DRA) is an intestinal Cl<sup>-</sup>/HCO<sub>3</sub><sup>-</sup> antiporter that is enriched in LRs. However, little is known regarding the mechanisms involved in the regulation of DRA activity. The air-liquid interface (ALI) was created by removing apical media for a specified number of days; from 12-14 days post-confluency, Caco-2/BBe cells or"],"journal":["International journal of molecular sciences"],"pubmed_title":["The Air-Liquid Interface Reorganizes Membrane Lipids and Enhances the Recruitment of Slc26a3 to Lipid-Rich Domains in Human Colonoid Monolayers."],"pmcid":["PMC10179158"],"funding_grant_id":["UO1-DK-10316","CF-SINGH20G0","R01-DK-116352","P30-DK-89502"],"pubmed_authors":["Lin R","Donowitz M","Zhang Z","Tse CM","Sarker R","Singh V"],"additional_accession":[]},"is_claimable":false,"name":"The Air-Liquid Interface Reorganizes Membrane Lipids and Enhances the Recruitment of Slc26a3 to Lipid-Rich Domains in Human Colonoid Monolayers.","description":"Cholesterol-rich membrane domains, also called lipid rafts (LRs), are specialized membrane domains that provide a platform for intracellular signal transduction. Membrane proteins often cluster in LRs that further aggregate into larger platform-like structures that are enriched in ceramides and are called ceramide-rich platforms (CRPs). The role of CRPs in the regulation of intestinal epithelial functions remains unknown. Down-regulated in adenoma (DRA) is an intestinal Cl<sup>-</sup>/HCO<sub>3</sub><sup>-</sup> antiporter that is enriched in LRs. However, little is known regarding the mechanisms involved in the regulation of DRA activity. The air-liquid interface (ALI) was created by removing apical media for a specified number of days; from 12-14 days post-confluency, Caco-2/BBe cells or","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2025-04-26T01:39:07.792Z","creation":"2025-04-06T10:13:29.888Z"},"accession":"S-EPMC10179158","cross_references":{"pubmed":["37175979"],"doi":["10.3390/ijms24098273"]}}