{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liu H"],"funding":["the National Science and Technology Major Project","the National Natural Science Foundation of China"],"pagination":["3942"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10179765"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28(9)"],"pubmed_abstract":["Cationic polymeric materials and cell-penetrating peptides (CPPs) were often used as the delivery vectors in the evaluation of nucleic acid therapeutics. 10-23 DNAzyme is a kind of potential antisense therapeutics by catalytic cleavage of the disease-related RNAs. Here, lipofectamine 2000 and Tat peptide were evaluated for their effect on the catalytic activity of 10-23 DNAzyme, with the observed rate constant, thermal stability, CD spectra, and PAGE analysis, with a duplex DNA mimicking DNAzyme-substrate as a control. It was shown that the cationic carriers had a negative effect on the catalytic performance of the 10-23 DNAzyme. Significantly, the destabilizing effect of the cationic carriers on the duplex formation was noteworthy, as a duplex formation is an essential prerequisite in the"],"journal":["Molecules (Basel, Switzerland)"],"pubmed_title":["Studies on the Effect of Lipofectamine and Cell-Penetrating Peptide on the Properties of 10-23 DNAzyme."],"pmcid":["PMC10179765"],"funding_grant_id":["21572268","2018ZX09711003-003-001"],"pubmed_authors":["Liu H","Li Y","Du S","He J","Wang C","Shi W","Liu S","Cao R"],"additional_accession":[]},"is_claimable":false,"name":"Studies on the Effect of Lipofectamine and Cell-Penetrating Peptide on the Properties of 10-23 DNAzyme.","description":"Cationic polymeric materials and cell-penetrating peptides (CPPs) were often used as the delivery vectors in the evaluation of nucleic acid therapeutics. 10-23 DNAzyme is a kind of potential antisense therapeutics by catalytic cleavage of the disease-related RNAs. Here, lipofectamine 2000 and Tat peptide were evaluated for their effect on the catalytic activity of 10-23 DNAzyme, with the observed rate constant, thermal stability, CD spectra, and PAGE analysis, with a duplex DNA mimicking DNAzyme-substrate as a control. It was shown that the cationic carriers had a negative effect on the catalytic performance of the 10-23 DNAzyme. Significantly, the destabilizing effect of the cationic carriers on the duplex formation was noteworthy, as a duplex formation is an essential prerequisite in the","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2025-04-21T22:47:37.343Z","creation":"2025-04-05T18:52:26.565Z"},"accession":"S-EPMC10179765","cross_references":{"pubmed":["37175352"],"doi":["10.3390/molecules28093942"]}}