biostudies-literatureKim YMinistry of SMEs and Startups3814https://www.ebi.ac.uk/biostudies/studies/S-EPMC10179809biostudies-literatureUnknown28(9)The pharmacological potential of industrial hemp (<i>Cannabis sativa</i>) has been widely studied. However, the majority of studies have focused on cannabidiol, isolated from the inflorescence and leaf of the plant. In the present study, we evaluated the anti-diabetic potential of hemp root water (HWE) and ethanol extracts (HEE) in streptozotocin (STZ)-induced insulin-deficient diabetic mice. The administration of HWE and HEE ameliorated hyperglycemia and improved glucose homeostasis and islet function in STZ-treated mice (<i>p</i> < 0.05). HWE and HEE suppressed β-cell apoptosis and cytokine-induced inflammatory signaling in the pancreas (<i>p</i> < 0.05). Moreover, HWE and HEE normalized insulin-signaling defects in skeletal muscles and apoptotic response in the liver and kidney induced by STZ (<i>p</i> < 0.05). Gas chromatography-mass spectrometry analysis of HWE and HEE showed possible active compounds which might be responsible for the observed anti-diabetic potential. These findings indicate the possible mechanisms by which hemp root extracts protect mice against insulin-deficient diabetes, and support the need for further studies geared towards the application of hemp root as a novel bioactive material.Molecules (Basel, Switzerland)Protective Effects of Hemp (<i>Cannabis sativa</i>) Root Extracts against Insulin-Deficient Diabetes Mellitus In Mice.PMC10179809P0016082Lee JBCho KHKim SHKwon GSKim WKim YSim KSKim JHKim KHfalseProtective Effects of Hemp (<i>Cannabis sativa</i>) Root Extracts against Insulin-Deficient Diabetes Mellitus In Mice.The pharmacological potential of industrial hemp (<i>Cannabis sativa</i>) has been widely studied. However, the majority of studies have focused on cannabidiol, isolated from the inflorescence and leaf of the plant. In the present study, we evaluated the anti-diabetic potential of hemp root water (HWE) and ethanol extracts (HEE) in streptozotocin (STZ)-induced insulin-deficient diabetic mice. The administration of HWE and HEE ameliorated hyperglycemia and improved glucose homeostasis and islet function in STZ-treated mice (<i>p</i> < 0.05). HWE and HEE suppressed β-cell apoptosis and cytokine-induced inflammatory signaling in the pancreas (<i>p</i> < 0.05). Moreover, HWE and HEE normalized insulin-signaling defects in skeletal muscles and apoptotic response in the liver and kidney induced by STZ (<i>p</i> < 0.05). Gas chromatography-mass spectrometry analysis of HWE and HEE showed possible active compounds which might be responsible for the observed anti-diabetic potential. These findings indicate the possible mechanisms by which hemp root extracts protect mice against insulin-deficient diabetes, and support the need for further studies geared towards the application of hemp root as a novel bioactive material.2023-01-01T00:00:00Z2023 Apr2025-04-21T22:44:28.988Z2025-04-05T18:50:53.816ZS-EPMC101798093717522410.3390/molecules28093814