biostudies-literatureVoronov ISRussian Science Foundation3945https://www.ebi.ac.uk/biostudies/studies/S-EPMC10180310biostudies-literatureUnknown28(9)Pentacyclic triterpenoids (PCTs), which possess a number of bioactive properties, are considered one of the most important classes of secondary plant metabolites. Their chromatographic determination in plant biomass is complicated by the need to separate a large number of structurally similar compounds belonging to several classes that differ greatly in polarity (monools, diols, and triterpenic acids). This study proposes a rapid, sensitive, and low-cost method for the simultaneous quantification of ten PCTs (3<i>β</i>-taraxerol, lupeol, <i>β</i>-amyrin, <i>α</i>-amyrin, betulin, erythrodiol, uvaol, betulinic, oleanolic, and ursolic acids) by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) using porous graphitic carbon (Hypercarb) as a stationary phase capable Molecules (Basel, Switzerland)Determination of Pentacyclic Triterpenoids in Plant Biomass by Porous Graphitic Carbon Liquid Chromatography-Tandem Mass Spectrometry.PMC1018031021-73-00291Voronov ISUl'yanovskii NVFaleva AVFalev DIKosyakov DSfalseDetermination of Pentacyclic Triterpenoids in Plant Biomass by Porous Graphitic Carbon Liquid Chromatography-Tandem Mass Spectrometry.Pentacyclic triterpenoids (PCTs), which possess a number of bioactive properties, are considered one of the most important classes of secondary plant metabolites. Their chromatographic determination in plant biomass is complicated by the need to separate a large number of structurally similar compounds belonging to several classes that differ greatly in polarity (monools, diols, and triterpenic acids). This study proposes a rapid, sensitive, and low-cost method for the simultaneous quantification of ten PCTs (3<i>β</i>-taraxerol, lupeol, <i>β</i>-amyrin, <i>α</i>-amyrin, betulin, erythrodiol, uvaol, betulinic, oleanolic, and ursolic acids) by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) using porous graphitic carbon (Hypercarb) as a stationary phase capable 2023-01-01T00:00:00Z2023 May2025-04-18T15:12:24.416Z2025-04-07T01:50:40.882ZS-EPMC101803103717535510.3390/molecules28093945