{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sajid A"],"funding":["Commonwealth Scholarship Commission"],"pagination":["2115"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10180698"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(9)"],"pubmed_abstract":["Chitosan is extensively studied as a carrier for gene delivery and is an attractive non-viral gene vector owing to its polycationic, biodegradable, and biocompatible nature. Thus, it is essential to understand the chemistry of self-assembled chitosan-DNA complexation and their structural and functional properties, enabling the formation of an effective non-viral gene delivery system. In this study, two parent chitosans (samples NAS-032 and NAS-075; Mw range ~118-164 kDa) and their depolymerised derivatives (deploy nas-032 and deploy nas-075; Mw range 6-14 kDa) with degrees of acetylation 43.4 and 4.7%, respectively, were used to form polyelectrolyte complexes (PECs) with DNA at varying [-NH<sub>3</sub><sup>+</sup>]/[-PO<sub>4</sub><sup>-</sup>] (N/P) molar charge ratios. We investigated th"],"journal":["Polymers"],"pubmed_title":["On the Fractionation and Physicochemical Characterisation of Self-Assembled Chitosan-DNA Polyelectrolyte Complexes."],"pmcid":["PMC10180698"],"funding_grant_id":["PKCS-2019-493"],"pubmed_authors":["Goycoolea FM","Sajid A","Castronovo M"],"additional_accession":[]},"is_claimable":false,"name":"On the Fractionation and Physicochemical Characterisation of Self-Assembled Chitosan-DNA Polyelectrolyte Complexes.","description":"Chitosan is extensively studied as a carrier for gene delivery and is an attractive non-viral gene vector owing to its polycationic, biodegradable, and biocompatible nature. Thus, it is essential to understand the chemistry of self-assembled chitosan-DNA complexation and their structural and functional properties, enabling the formation of an effective non-viral gene delivery system. In this study, two parent chitosans (samples NAS-032 and NAS-075; Mw range ~118-164 kDa) and their depolymerised derivatives (deploy nas-032 and deploy nas-075; Mw range 6-14 kDa) with degrees of acetylation 43.4 and 4.7%, respectively, were used to form polyelectrolyte complexes (PECs) with DNA at varying [-NH<sub>3</sub><sup>+</sup>]/[-PO<sub>4</sub><sup>-</sup>] (N/P) molar charge ratios. We investigated th","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2025-04-05T10:54:34.461Z","creation":"2025-04-05T10:54:34.461Z"},"accession":"S-EPMC10180698","cross_references":{"pubmed":["37177260"],"doi":["10.3390/polym15092115"]}}