biostudies-literatureXu JProgram of Processing and Efficient Utilization of Biomass Resources of Henan Center for Out-standing Overseas ScientistsNational Natural Science Foundation of ChinaProgram for Science & Technology Innovative Research Team in the University of Henan ProvinceKey Program of Henan Provincial Science and Technology R&D Plan Joint Fund for Cultivation of Superior DisciplinesYoung Elite Scientists Sponsorship Program by Henan Association for Science and TechnologyInnovation Leadership Program in Sciences and Technologies for Central Plains Talent PlanHenan Provincial Key Research and Development Program1991https://www.ebi.ac.uk/biostudies/studies/S-EPMC10180774biostudies-literatureUnknown15(9)A water-soluble cationic kraft lignin (named JLQKL₅₀), synthesized by combining quaternization and crosslinking reactions, was used as an additive to enhance the enzymatic hydrolysis of dilute-alkali-pretreated corn stalk. The chemical constitution of JLQKL₅₀ was investigated by Fourier transform infrared spectroscopy, ¹H nuclear magnetic resonance (NMR) and ¹³C NMR spectroscopy, and elemental analysis. The enzymatic hydrolysis efficiency of corn stalk at solid content of 10% (<i>w</i>/<i>v</i>) was significantly improved from 70.67% to 78.88% after 24 h when JLQKL₅₀ was added at a concentration of 2 g/L. Meanwhile, the enzymatic hydrolysis efficiency after 72 h reached 91.11% with 10 FPU/g of cellulase and 97.92% with 15 FPU/g of cellPolymersEmploying Cationic Kraft Lignin as Additive to Enhance Enzymatic Hydrolysis of Corn Stalk.PMC10180774202102210312 and 2121023108652022HYTP018 and 2021HYTP02222IRTSTHN00722278382, 21908205, 22078308, and 22208324214200510009222301420008GZS2022007Li HAlam MAZhao ALv YMuhammad GZhang SXu JXiong WfalseEmploying Cationic Kraft Lignin as Additive to Enhance Enzymatic Hydrolysis of Corn Stalk.A water-soluble cationic kraft lignin (named JLQKL₅₀), synthesized by combining quaternization and crosslinking reactions, was used as an additive to enhance the enzymatic hydrolysis of dilute-alkali-pretreated corn stalk. The chemical constitution of JLQKL₅₀ was investigated by Fourier transform infrared spectroscopy, ¹H nuclear magnetic resonance (NMR) and ¹³C NMR spectroscopy, and elemental analysis. The enzymatic hydrolysis efficiency of corn stalk at solid content of 10% (<i>w</i>/<i>v</i>) was significantly improved from 70.67% to 78.88% after 24 h when JLQKL₅₀ was added at a concentration of 2 g/L. Meanwhile, the enzymatic hydrolysis efficiency after 72 h reached 91.11% with 10 FPU/g of cellulase and 97.92% with 15 FPU/g of cell2023-01-01T00:00:00Z2023 Apr2025-04-25T22:37:20.201Z2025-04-06T09:05:14.681ZS-EPMC101807743717713910.3390/polym15091991