biostudies-literatureRacz GABudapest University of Technology and EconomicsNational Research, Development and Innovation Office7760https://www.ebi.ac.uk/biostudies/studies/S-EPMC10181998biostudies-literatureUnknown13(1)In human cells two dUTPase isoforms have been described: one nuclear (DUT-N) and one mitochondrial (DUT-M), with cognate localization signals. In contrast, here we identified two additional isoforms; DUT-3 without any localization signal and DUT-4 with the same nuclear localization signal as DUT-N. Based on an RT-qPCR method for simultaneous isoform-specific quantification we analysed the relative expression patterns in 20 human cell lines of highly different origins. We found that the DUT-N isoform is expressed by far at the highest level, followed by the DUT-M and the DUT-3 isoform. A strong correlation between expression levels of DUT-M and DUT-3 suggests that these two isoforms may share the same promoter. We analysed the effect of serum starvation on the expression of dUTPase isoformsScientific reportsDiscovery of two new isoforms of the human DUT gene.PMC10181998TKP2020-NKA-26OTKA-K128011OTKA-K128369K119493Nagy NRacz GAVarady GTovari JApati AVertessy BGfalseDiscovery of two new isoforms of the human DUT gene.In human cells two dUTPase isoforms have been described: one nuclear (DUT-N) and one mitochondrial (DUT-M), with cognate localization signals. In contrast, here we identified two additional isoforms; DUT-3 without any localization signal and DUT-4 with the same nuclear localization signal as DUT-N. Based on an RT-qPCR method for simultaneous isoform-specific quantification we analysed the relative expression patterns in 20 human cell lines of highly different origins. We found that the DUT-N isoform is expressed by far at the highest level, followed by the DUT-M and the DUT-3 isoform. A strong correlation between expression levels of DUT-M and DUT-3 suggests that these two isoforms may share the same promoter. We analysed the effect of serum starvation on the expression of dUTPase isoforms2023-01-01T00:00:00Z2023 May2026-05-28T22:49:08.033Z2024-11-20T23:13:26.171ZS-EPMC101819983717333710.1038/s41598-023-32970-1