{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Vergara-Barberan M"],"funding":["Universitat de Valencia","Ministerio de Ciencia e Innovación"],"pagination":["219"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10182934"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["190(6)"],"pubmed_abstract":["An aptamer-functionalized stir bar sorptive extraction (SBSE) coating is described for the first time devoted to selective isolation and preconcentration of an allergenic food protein, concavanalin A (Con A), followed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS) determination. For this purpose, the polytetrafluoroethylene surface of commercial magnetic stir bars was properly modified and vinylized to immobilize a thiol-modified aptamer against Con A via straightforward \"thiol-ene\" click chemistry. The aptamer-functionalized stir bar was employed as SBSE sorbent to isolate Con A, and several parameters that can affect the extraction efficiency were investigated. Under the optimized conditions, Con A was extracted and desorbed during 30 and 45 min, respecti"],"journal":["Mikrochimica acta"],"pubmed_title":["Aptamer-functionalized stir bar sorptive extraction for selective isolation, identification, and determination of concanavalin A in food by MALDI-TOF-MS."],"pmcid":["PMC10182934"],"funding_grant_id":["PID2021-127137OB-I00","PID2021-125459OB-I00"],"pubmed_authors":["Herrero-Martinez JM","Catala-Icardo M","Benavente F","Vergara-Barberan M","Simo-Alfonso EF"],"additional_accession":[]},"is_claimable":false,"name":"Aptamer-functionalized stir bar sorptive extraction for selective isolation, identification, and determination of concanavalin A in food by MALDI-TOF-MS.","description":"An aptamer-functionalized stir bar sorptive extraction (SBSE) coating is described for the first time devoted to selective isolation and preconcentration of an allergenic food protein, concavanalin A (Con A), followed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS) determination. For this purpose, the polytetrafluoroethylene surface of commercial magnetic stir bars was properly modified and vinylized to immobilize a thiol-modified aptamer against Con A via straightforward \"thiol-ene\" click chemistry. The aptamer-functionalized stir bar was employed as SBSE sorbent to isolate Con A, and several parameters that can affect the extraction efficiency were investigated. Under the optimized conditions, Con A was extracted and desorbed during 30 and 45 min, respecti","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2025-04-19T03:34:11.978Z","creation":"2025-04-07T13:42:35.651Z"},"accession":"S-EPMC10182934","cross_references":{"pubmed":["37178355"],"doi":["10.1007/s00604-023-05795-y"]}}