biostudies-literatureUnknown15(2)Eslami M<h4>Background and objectives</h4>Previous researchers showed the antimicrobial ability of ionic liquids (ILs) on different infective agents. ILs can dissolve organic components, especially DNA molecules. Among synthesized eight binary ILs mixtures, we have chosen ([Met-HCl] [PyS]) IL for determining the antifungal ability of IL against <i>Candida albicans</i> cells.<h4>Materials and methods</h4>Well diffusion assay, chrome agar and Germ tube tests were used to detect the <i>Candida</i> samples. PCR, real-time-PCR, and flow cytometry tests were performed to determine the IL's rate of toxic ability.<h4>Results</h4>Well diffusion assay revealed the diameters of the growth inhibition zones were the largest in IL with methionine and Proline amino acids. Minimum inhibitory concentration (MIC) aIranian journal of microbiology325-335https://www.ebi.ac.uk/biostudies/studies/S-EPMC10183073biostudies-literatureEffect of new methionine-based ionic liquid on the <i>CDR1</i> and <i>CDR2</i> gene expression on sensitive and resistant strains of <i>Candida albicans</i>.PMC10183073Omidi BBayat MMortazavi PEslami MEzabadi AfalseEffect of new methionine-based ionic liquid on the <i>CDR1</i> and <i>CDR2</i> gene expression on sensitive and resistant strains of <i>Candida albicans</i>.<h4>Background and objectives</h4>Previous researchers showed the antimicrobial ability of ionic liquids (ILs) on different infective agents. ILs can dissolve organic components, especially DNA molecules. Among synthesized eight binary ILs mixtures, we have chosen ([Met-HCl] [PyS]) IL for determining the antifungal ability of IL against <i>Candida albicans</i> cells.<h4>Materials and methods</h4>Well diffusion assay, chrome agar and Germ tube tests were used to detect the <i>Candida</i> samples. PCR, real-time-PCR, and flow cytometry tests were performed to determine the IL's rate of toxic ability.<h4>Results</h4>Well diffusion assay revealed the diameters of the growth inhibition zones were the largest in IL with methionine and Proline amino acids. Minimum inhibitory concentration (MIC) a2023-01-01T00:00:00Z2023 Apr2025-04-04T13:07:24.449Z2025-04-04T13:07:24.449ZS-EPMC101830733719323810.18502/ijm.v15i2.12485