{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["29(1)"],"submitter":["Mijiti M"],"pubmed_abstract":["<h4>Background</h4>Low-grade gliomas (LGG) are a type of brain tumor that can be lethal, and it is essential to identify genes that are correlated with patient prognosis. In this study, we aimed to use CRISPR-cas9 screening data to identify key signaling pathways and develop a genetic signature associated with high-risk, low-grade glioma patients.<h4>Methods</h4>The study used CRISPR-cas9 screening data to identify essential genes correlated with cell survival in LGG. We used RNA-seq data to identify differentially expressed genes (DEGs) related to cell viability. Moreover, we used the least absolute shrinkage and selection operator (LASSO) method to construct a genetic signature for predicting overall survival in patients. We performed enrichment analysis to identify pathways mediated by "],"journal":["Molecular medicine (Cambridge, Mass.)"],"pagination":["64"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10183247"],"repository":["biostudies-literature"],"pubmed_title":["CRISPR-cas9 screening identified lethal genes enriched in Hippo kinase pathway and of predictive significance in primary low-grade glioma."],"pmcid":["PMC10183247"],"pubmed_authors":["Yiming N","Dilixiati Y","Zhu G","Tuersun M","Kasimu M","Dilixiati M","Wu H","Abulaiti A","Li Y","Turhon M","Mijiti M","Chen X","Maimaitiaili N","Wang Y","Maimaiti A"],"additional_accession":[]},"is_claimable":false,"name":"CRISPR-cas9 screening identified lethal genes enriched in Hippo kinase pathway and of predictive significance in primary low-grade glioma.","description":"<h4>Background</h4>Low-grade gliomas (LGG) are a type of brain tumor that can be lethal, and it is essential to identify genes that are correlated with patient prognosis. In this study, we aimed to use CRISPR-cas9 screening data to identify key signaling pathways and develop a genetic signature associated with high-risk, low-grade glioma patients.<h4>Methods</h4>The study used CRISPR-cas9 screening data to identify essential genes correlated with cell survival in LGG. We used RNA-seq data to identify differentially expressed genes (DEGs) related to cell viability. Moreover, we used the least absolute shrinkage and selection operator (LASSO) method to construct a genetic signature for predicting overall survival in patients. We performed enrichment analysis to identify pathways mediated by ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2025-04-22T08:48:27.666Z","creation":"2025-04-05T22:41:12.369Z"},"accession":"S-EPMC10183247","cross_references":{"pubmed":["37183261"],"doi":["10.1186/s10020-023-00652-3"]}}