biostudies-literatureUnknown29(1)Mijiti M<h4>Background</h4>Low-grade gliomas (LGG) are a type of brain tumor that can be lethal, and it is essential to identify genes that are correlated with patient prognosis. In this study, we aimed to use CRISPR-cas9 screening data to identify key signaling pathways and develop a genetic signature associated with high-risk, low-grade glioma patients.<h4>Methods</h4>The study used CRISPR-cas9 screening data to identify essential genes correlated with cell survival in LGG. We used RNA-seq data to identify differentially expressed genes (DEGs) related to cell viability. Moreover, we used the least absolute shrinkage and selection operator (LASSO) method to construct a genetic signature for predicting overall survival in patients. We performed enrichment analysis to identify pathways mediated by Molecular medicine (Cambridge, Mass.)64https://www.ebi.ac.uk/biostudies/studies/S-EPMC10183247biostudies-literatureCRISPR-cas9 screening identified lethal genes enriched in Hippo kinase pathway and of predictive significance in primary low-grade glioma.PMC10183247Yiming NDilixiati YZhu GTuersun MKasimu MDilixiati MWu HAbulaiti ALi YTurhon MMijiti MChen XMaimaitiaili NWang YMaimaiti AfalseCRISPR-cas9 screening identified lethal genes enriched in Hippo kinase pathway and of predictive significance in primary low-grade glioma.<h4>Background</h4>Low-grade gliomas (LGG) are a type of brain tumor that can be lethal, and it is essential to identify genes that are correlated with patient prognosis. In this study, we aimed to use CRISPR-cas9 screening data to identify key signaling pathways and develop a genetic signature associated with high-risk, low-grade glioma patients.<h4>Methods</h4>The study used CRISPR-cas9 screening data to identify essential genes correlated with cell survival in LGG. We used RNA-seq data to identify differentially expressed genes (DEGs) related to cell viability. Moreover, we used the least absolute shrinkage and selection operator (LASSO) method to construct a genetic signature for predicting overall survival in patients. We performed enrichment analysis to identify pathways mediated by 2023-01-01T00:00:00Z2023 May2025-04-22T08:48:27.666Z2025-04-05T22:41:12.369ZS-EPMC101832473718326110.1186/s10020-023-00652-3