biostudies-literatureChen XNational Natural Science Foundation of China2208707https://www.ebi.ac.uk/biostudies/studies/S-EPMC10184595biostudies-literatureUnknown18(1)Glutathione peroxidase 8 (GPX8) is a key regulator of redox homoeostasis. Whether its antioxidant activity participates in the regulation of m⁶A modification is a crucial issue, which has important application value in cancer treatment. In this study, MeRIP-seq was used to explore the characteristics of transcriptome-wide m⁶A modification in GPX8-deficient oral cancer cells. Oxidative stress caused by the lack of GPX8 resulted in 1,279 hyper- and 2,287 hypo-methylated m⁶A peaks and 2,036 differentially expressed genes in GPX8-KO cells. Twenty-eight differentially expressed genes were related to the cell response to oxidative stress, and half of them changed their m⁶A modification. In GPX8-KO cells, m⁶A regulators IGF2BP2 and IGF2BP3 weEpigeneticsGPX8 deficiency-induced oxidative stress reprogrammed m6A epitranscriptome of oral cancer cells.PMC1018459581873711Wu JHe YWang CChen LYuan LZhao WYu DChen XZhang LChen SfalseGPX8 deficiency-induced oxidative stress reprogrammed m6A epitranscriptome of oral cancer cells.Glutathione peroxidase 8 (GPX8) is a key regulator of redox homoeostasis. Whether its antioxidant activity participates in the regulation of m⁶A modification is a crucial issue, which has important application value in cancer treatment. In this study, MeRIP-seq was used to explore the characteristics of transcriptome-wide m⁶A modification in GPX8-deficient oral cancer cells. Oxidative stress caused by the lack of GPX8 resulted in 1,279 hyper- and 2,287 hypo-methylated m⁶A peaks and 2,036 differentially expressed genes in GPX8-KO cells. Twenty-eight differentially expressed genes were related to the cell response to oxidative stress, and half of them changed their m⁶A modification. In GPX8-KO cells, m⁶A regulators IGF2BP2 and IGF2BP3 we2023-01-01T00:00:00Z2023 Dec2025-04-25T22:33:29.563Z2025-04-06T09:03:59.741ZS-EPMC101845953717059110.1080/15592294.2023.2208707