{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["19(4)"],"submitter":["Aigha II"],"funding":["Hamad bin Khalifa University"],"pubmed_abstract":["The multipotent pancreatic progenitor cells (MPCs) co-expressing the transcription factors, PDX1 and NKX6.1, are the source of functional pancreatic β-cells. The aim of this study was to examine the effect of p53 inhibition in MPCs on the generation of PDX1<sup>+</sup>/NKX6.1<sup>+</sup> MPCs and pancreatic β-cell generation. Human embryonic stem cells (hESCs) were differentiated into MPCs and β-cells. hESC-MPCs (stage 4) were treated with different concentrations of p53 inhibitors, and their effect was evaluated using different approaches. NKX6.1 was overexpressed during MPCs specification. Inhibition of p53 using pifithrin-μ (PFT-μ) at the MPC stage resulted in a significant increase in the number of PDX1<sup>+</sup>/NKX6.1<sup>+</sup> cells and a reduction in the number of CHGA<sup>+</s"],"journal":["Stem cell reviews and reports"],"pagination":["942-952"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10185607"],"repository":["biostudies-literature"],"pubmed_title":["p53 Inhibition in Pancreatic Progenitors Enhances the Differentiation of Human Pluripotent Stem Cells into Pancreatic β-Cells."],"pmcid":["PMC10185607"],"pubmed_authors":["Abdelalim EM","Aigha II"],"additional_accession":[]},"is_claimable":false,"name":"p53 Inhibition in Pancreatic Progenitors Enhances the Differentiation of Human Pluripotent Stem Cells into Pancreatic β-Cells.","description":"The multipotent pancreatic progenitor cells (MPCs) co-expressing the transcription factors, PDX1 and NKX6.1, are the source of functional pancreatic β-cells. The aim of this study was to examine the effect of p53 inhibition in MPCs on the generation of PDX1<sup>+</sup>/NKX6.1<sup>+</sup> MPCs and pancreatic β-cell generation. Human embryonic stem cells (hESCs) were differentiated into MPCs and β-cells. hESC-MPCs (stage 4) were treated with different concentrations of p53 inhibitors, and their effect was evaluated using different approaches. NKX6.1 was overexpressed during MPCs specification. Inhibition of p53 using pifithrin-μ (PFT-μ) at the MPC stage resulted in a significant increase in the number of PDX1<sup>+</sup>/NKX6.1<sup>+</sup> cells and a reduction in the number of CHGA<sup>+</s","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2025-04-22T08:48:38.407Z","creation":"2025-04-05T22:41:15.997Z"},"accession":"S-EPMC10185607","cross_references":{"pubmed":["36707464"],"doi":["10.1007/s12015-023-10509-1"]}}