{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Koster KA"],"funding":["Deutsche Forschungsgemeinschaft","Universitätsklinikum Hamburg-Eppendorf (UKE)"],"pagination":["1223-1233"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10185614"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["396(6)"],"pubmed_abstract":["The dual leucine zipper kinase (DLK) and the ubiquitously expressed transcription factor c-FOS have important roles in beta-cell proliferation and function. Some studies in neuronal cells suggest that DLK can influence c-FOS expression. Given that c-FOS is mainly regulated at the transcriptional level, the effect of DLK on c-FOS promoter activity was investigated in the beta-cell line HIT. The methods used in this study are the following: Luciferase reporter gene assays, immunoblot analysis, CRISPR-Cas9-mediated genome editing, and real-time quantitative PCR. In the beta-cell line HIT, overexpressed DLK increased c-FOS promoter activity twofold. Using 5'-,3'-promoter deletions, the promoter regions from - 348 to - 339 base pairs (bp) and from a - 284 to - 53 bp conferred basal activity, wh"],"journal":["Naunyn-Schmiedeberg's archives of pharmacology"],"pubmed_title":["Increase of c-FOS promoter transcriptional activity by the dual leucine zipper kinase."],"pmcid":["PMC10185614"],"funding_grant_id":["OE 181/5-1"],"pubmed_authors":["Duque Escobar J","Toledo R","Oetjen E","Fietkau A","Koster KA"],"additional_accession":[]},"is_claimable":false,"name":"Increase of c-FOS promoter transcriptional activity by the dual leucine zipper kinase.","description":"The dual leucine zipper kinase (DLK) and the ubiquitously expressed transcription factor c-FOS have important roles in beta-cell proliferation and function. Some studies in neuronal cells suggest that DLK can influence c-FOS expression. Given that c-FOS is mainly regulated at the transcriptional level, the effect of DLK on c-FOS promoter activity was investigated in the beta-cell line HIT. The methods used in this study are the following: Luciferase reporter gene assays, immunoblot analysis, CRISPR-Cas9-mediated genome editing, and real-time quantitative PCR. In the beta-cell line HIT, overexpressed DLK increased c-FOS promoter activity twofold. Using 5'-,3'-promoter deletions, the promoter regions from - 348 to - 339 base pairs (bp) and from a - 284 to - 53 bp conferred basal activity, wh","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2025-04-26T17:23:44.237Z","creation":"2025-04-06T15:33:19.22Z"},"accession":"S-EPMC10185614","cross_references":{"pubmed":["36700987"],"doi":["10.1007/s00210-023-02401-z"]}}