biostudies-literatureKoster KADeutsche ForschungsgemeinschaftUniversitätsklinikum Hamburg-Eppendorf (UKE)1223-1233https://www.ebi.ac.uk/biostudies/studies/S-EPMC10185614biostudies-literatureUnknown396(6)The dual leucine zipper kinase (DLK) and the ubiquitously expressed transcription factor c-FOS have important roles in beta-cell proliferation and function. Some studies in neuronal cells suggest that DLK can influence c-FOS expression. Given that c-FOS is mainly regulated at the transcriptional level, the effect of DLK on c-FOS promoter activity was investigated in the beta-cell line HIT. The methods used in this study are the following: Luciferase reporter gene assays, immunoblot analysis, CRISPR-Cas9-mediated genome editing, and real-time quantitative PCR. In the beta-cell line HIT, overexpressed DLK increased c-FOS promoter activity twofold. Using 5'-,3'-promoter deletions, the promoter regions from - 348 to - 339 base pairs (bp) and from a - 284 to - 53 bp conferred basal activity, whNaunyn-Schmiedeberg's archives of pharmacologyIncrease of c-FOS promoter transcriptional activity by the dual leucine zipper kinase.PMC10185614OE 181/5-1Duque Escobar JToledo ROetjen EFietkau AKoster KAfalseIncrease of c-FOS promoter transcriptional activity by the dual leucine zipper kinase.The dual leucine zipper kinase (DLK) and the ubiquitously expressed transcription factor c-FOS have important roles in beta-cell proliferation and function. Some studies in neuronal cells suggest that DLK can influence c-FOS expression. Given that c-FOS is mainly regulated at the transcriptional level, the effect of DLK on c-FOS promoter activity was investigated in the beta-cell line HIT. The methods used in this study are the following: Luciferase reporter gene assays, immunoblot analysis, CRISPR-Cas9-mediated genome editing, and real-time quantitative PCR. In the beta-cell line HIT, overexpressed DLK increased c-FOS promoter activity twofold. Using 5'-,3'-promoter deletions, the promoter regions from - 348 to - 339 base pairs (bp) and from a - 284 to - 53 bp conferred basal activity, wh2023-01-01T00:00:00Z2023 Jun2025-04-26T17:23:44.237Z2025-04-06T15:33:19.22ZS-EPMC101856143670098710.1007/s00210-023-02401-z