biostudies-literatureHuang Jthe Yichun Dahaigui Life Science Co., Ltdthe Hubei Hongshan Laboratorythe National Natural Science Foundation of China430-441https://www.ebi.ac.uk/biostudies/studies/S-EPMC10186138biostudies-literatureUnknown24(5)Early weaned piglets suffer from oxidative stress and enteral infection, which usually results in gut microbial dysbiosis, serve diarrhea, and even death. Rice bran oil (RBO), a polyphenol-enriched by-product of rice processing, has been shown to have antioxidant and anti-inflammatory properties both in vivo and in vitro. Here, we ascertained the proper RBO supplementation level, and subsequently determined its effects on lipopolysaccharide (LPS)-induced intestinal dysfunction in weaned piglets. A total of 168 piglets were randomly allocated into four groups of seven replicates (42 piglets each group, (21±1) d of age, body weight (7.60±0.04) kg, and half males and half females) and were given basal diet (Ctrl) or basal diet supplemented with 0.01% (mass fraction) RBO (RBO1), 0.02% RBO (RBOJournal of Zhejiang University. Science. BRice bran oil supplementation protects swine weanlings against diarrhea and lipopolysaccharide challenge.PMC1018613832230099 and 319250372021hszd01820222ZDH04093Ma LHuang JXu BChen YQin WSun HZhao JYan XJing FXu YfalseRice bran oil supplementation protects swine weanlings against diarrhea and lipopolysaccharide challenge.Early weaned piglets suffer from oxidative stress and enteral infection, which usually results in gut microbial dysbiosis, serve diarrhea, and even death. Rice bran oil (RBO), a polyphenol-enriched by-product of rice processing, has been shown to have antioxidant and anti-inflammatory properties both in vivo and in vitro. Here, we ascertained the proper RBO supplementation level, and subsequently determined its effects on lipopolysaccharide (LPS)-induced intestinal dysfunction in weaned piglets. A total of 168 piglets were randomly allocated into four groups of seven replicates (42 piglets each group, (21±1) d of age, body weight (7.60±0.04) kg, and half males and half females) and were given basal diet (Ctrl) or basal diet supplemented with 0.01% (mass fraction) RBO (RBO1), 0.02% RBO (RBO2023-01-01T00:00:00Z2023 May2025-04-25T22:30:43.643Z2025-04-06T09:03:33.396ZS-EPMC101861383719089210.1631/jzus.b220056510.1631/jzus.B2200565