{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Hofman DA"],"funding":["British Heart Foundation","NCI NIH HHS"],"pubmed_abstract":["A hallmark of high-risk childhood medulloblastoma is the dysregulation of RNA translation. Currently, it is unknown whether medulloblastoma dysregulates the translation of putatively oncogenic non-canonical open reading frames. To address this question, we performed ribosome profiling of 32 medulloblastoma tissues and cell lines and observed widespread non-canonical ORF translation. We then developed a step-wise approach to employ multiple CRISPR-Cas9 screens to elucidate functional non-canonical ORFs implicated in medulloblastoma cell survival. We determined that multiple lncRNA-ORFs and upstream open reading frames (uORFs) exhibited selective functionality independent of the main coding sequence. One of these, ASNSD1-uORF or ASDURF, was upregulated, associated with the MYC family oncogen"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2023.05.04.539399"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10187264"],"repository":["biostudies-literature"],"pubmed_title":["Translation of non-canonical open reading frames as a cancer cell survival mechanism in childhood medulloblastoma."],"pmcid":["PMC10187264"],"funding_grant_id":["SP/19/1/34461","R35 CA242457","K08 CA263552"],"pubmed_authors":["Brown A","Goodale A","Ligon KL","Hofman DA","Condurat AL","Root DE","van Dinter JT","Clauser KR","Phoenix TN","Prensner JR","van Heesch S","Wang L","Bandopadhayay P","Abid T","Hubner N","Murugesan R","Ruiz-Orera J","Zhou KN","Engels SAG","van der Lugt J","Yannuzzi I","Vogelzang J","Roth JA","Golub TR"],"additional_accession":[]},"is_claimable":false,"name":"Translation of non-canonical open reading frames as a cancer cell survival mechanism in childhood medulloblastoma.","description":"A hallmark of high-risk childhood medulloblastoma is the dysregulation of RNA translation. Currently, it is unknown whether medulloblastoma dysregulates the translation of putatively oncogenic non-canonical open reading frames. To address this question, we performed ribosome profiling of 32 medulloblastoma tissues and cell lines and observed widespread non-canonical ORF translation. We then developed a step-wise approach to employ multiple CRISPR-Cas9 screens to elucidate functional non-canonical ORFs implicated in medulloblastoma cell survival. We determined that multiple lncRNA-ORFs and upstream open reading frames (uORFs) exhibited selective functionality independent of the main coding sequence. One of these, ASNSD1-uORF or ASDURF, was upregulated, associated with the MYC family oncogen","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2026-04-08T13:54:51.172Z","creation":"2025-02-19T04:20:44.244Z"},"accession":"S-EPMC10187264","cross_references":{"pubmed":["37205492"],"doi":["10.1101/2023.05.04.539399"]}}