biostudies-literatureUnknownTrinidad CHigh grade serous ovarian carcinoma (HGSOC) accounts for ~ 70% of ovarian cancer cases. Non-invasive, highly specific blood-based tests for pre-symptomatic screening in women are crucial to reducing the mortality associated with this disease. Since most HGSOCs typically arise from the fallopian tubes (FT), our biomarker search focused on proteins found on the surface of extracellular vesicles (EVs) released by both FT and HGSOC tissue explants and representative cell lines. Using mass spectrometry, 985 EV proteins (exo-proteins) were identified that comprised the FT/HGSOC EV core proteome. Transmembrane exo-proteins were prioritized because these could serve as antigens for capture and/or detection. With a nano-engineered microfluidic platform, six newly discovered exo-proteins (ACSL4, IGSResearch squarers.3.rs-2814022https://www.ebi.ac.uk/biostudies/studies/S-EPMC10187430biostudies-literatureLineage specific extracellular vesicle-associated protein biomarkers for the early detection of high grade serous ovarian cancer.PMC10187430Cheng SSardiu MTrinidad CPathak HDeighan CJewell ABantis LTzeng SCMadan RZeng YGodwin AfalseLineage specific extracellular vesicle-associated protein biomarkers for the early detection of high grade serous ovarian cancer.High grade serous ovarian carcinoma (HGSOC) accounts for ~ 70% of ovarian cancer cases. Non-invasive, highly specific blood-based tests for pre-symptomatic screening in women are crucial to reducing the mortality associated with this disease. Since most HGSOCs typically arise from the fallopian tubes (FT), our biomarker search focused on proteins found on the surface of extracellular vesicles (EVs) released by both FT and HGSOC tissue explants and representative cell lines. Using mass spectrometry, 985 EV proteins (exo-proteins) were identified that comprised the FT/HGSOC EV core proteome. Transmembrane exo-proteins were prioritized because these could serve as antigens for capture and/or detection. With a nano-engineered microfluidic platform, six newly discovered exo-proteins (ACSL4, IGS2023-01-01T00:00:00Z2023 May2025-04-19T17:19:34.311Z2025-02-19T03:26:16.457ZS-EPMC101874303720557310.21203/rs.3.rs-2814022/v1