biostudies-literatureUnknown51(6)Peronnet E<h4>Objectives</h4>There is a crucial unmet need for biomarker-guided diagnostic and prognostic enrichment in clinical trials evaluating immune modulating therapies in critically ill patients. Low monocyte expression of human leukocyte antigen-DR (mHLA-DR), considered as a reference surrogate to identify immunosuppressed patients, has been proposed for patient stratification in immunostimulation approaches. However, its widespread use in clinic has been somewhat hampered by technical constraints inherent to flow cytometry technology. The objective of the present study was to evaluate the ability of a prototype multiplex polymerase chain reaction tool (immune profiling panel [IPP]) to identify immunosuppressed ICU patients characterized by a low mHLA-DR expression.<h4>Design</h4>RetrospectiCritical care medicine808-816https://www.ebi.ac.uk/biostudies/studies/S-EPMC10187625biostudies-literatureImmune Profiling Panel Gene Set Identifies Critically Ill Patients With Low Monocyte Human Leukocyte Antigen-DR Expression: Preliminary Results From the REAnimation Low Immune Status Marker (REALISM) Study.PMC10187625Gossez MCarre CBequet FGardiner DCaruso LRaychaudhuri AMartin OTissot SOriol GTruc CKoenig NTextoris JCrozon-Clauzel JBounab YHuriaux LJourdan CMallet FDebord-Peguet SGregoire AMommert MGatel JUberti TRiffaud CBerger SBDevigne BVallin HMaucort-Boulch DGenin CFlorin KBrengel-Pesce KMoriceau FConti FGallet-Gorius EMeuret PMacnamara AMoriniere JPeleraux AHengy BGillespie IToetsch SAugris-Mathieu CCollus MArnal SDelsuc CLouis KTabone OCanard BRimmele Tde Monfort AMoucade VBenatir FTran TBen-Amor ACerro VLepape ASchrevel CMouscaz YBrajon FSmylie RPerret MDelwarde BJadaud CMonneret GTerraz GMonard CGough PJPayrard SPachot ARol MLReynier FMeunier BMistretta MAshton TPeronnet ETan LBertin-Maghit MFugier IPanel NGorse IGarcon NLeissner PSapay NCejka JCRahali NFaure ACortez PFraisse LBlein SMarcotte GBertin-Maghit ABouffard YDavidson JLeray VVedrine CKreitmann LREAnimation Low Immune Status Marker (REALISM) Study GroupBecker JLukaszewicz ACMatray MCheynet VGirardot TFayolle-Pivot LBer CEDeleat-Besson RLlitjos JFGuyard CNoailles LLavocat FCerrato EBayle FQuemeneur LBellocq ASMarcos JYBoibieux AZadam AFleurie AGriffiths AFloccard BGuichard AVenet FTipple CfalseImmune Profiling Panel Gene Set Identifies Critically Ill Patients With Low Monocyte Human Leukocyte Antigen-DR Expression: Preliminary Results From the REAnimation Low Immune Status Marker (REALISM) Study.<h4>Objectives</h4>There is a crucial unmet need for biomarker-guided diagnostic and prognostic enrichment in clinical trials evaluating immune modulating therapies in critically ill patients. Low monocyte expression of human leukocyte antigen-DR (mHLA-DR), considered as a reference surrogate to identify immunosuppressed patients, has been proposed for patient stratification in immunostimulation approaches. However, its widespread use in clinic has been somewhat hampered by technical constraints inherent to flow cytometry technology. The objective of the present study was to evaluate the ability of a prototype multiplex polymerase chain reaction tool (immune profiling panel [IPP]) to identify immunosuppressed ICU patients characterized by a low mHLA-DR expression.<h4>Design</h4>Retrospecti2023-01-01T00:00:00Z2023 Jun2026-06-11T05:48:41.605Z2025-02-19T00:12:23.733ZS-EPMC101876253691759410.1097/CCM.0000000000005832