biostudies-literatureSanchez AMNSFNCI NIH HHSNational Institutes of HealthNIHNIGMS NIH HHSNational Science Foundation808-825https://www.ebi.ac.uk/biostudies/studies/S-EPMC10187668biostudies-literatureUnknown29(6)Fission yeast phosphate homeostasis gene <i>pho1</i> is actively repressed during growth in phosphate-rich medium by transcription in <i>cis</i> of a long noncoding (lnc) RNA from the 5' flanking <i>prt(nc-pho1)</i> gene. Pho1 expression is: (i) derepressed by genetic maneuvers that favor precocious lncRNA 3'-processing and termination, in response to DSR and PAS signals in <i>prt</i>; and (ii) hyperrepressed in genetic backgrounds that dampen 3'-processing/termination efficiency. Governors of 3'-processing/termination include the RNA polymerase CTD code, the CPF (cleavage and polyadenylation factor) complex, termination factors Seb1 and Rhn1, and the inositol pyrophosphate signaling molecule 1,5-IP₈ Here, we present genetic and biochemical evidence that fission yeast Duf89, a mRNA (New York, N.Y.)Duf89 abets lncRNA control of fission yeast phosphate homeostasis via its antagonism of precocious lncRNA transcription termination.PMC10187668R35 GM126945P30 CA008748R35-GM126945R01-GM1340211746057R01 GM134021Schwer BShuman SGarg ASanchez AMfalseDuf89 abets lncRNA control of fission yeast phosphate homeostasis via its antagonism of precocious lncRNA transcription termination.Fission yeast phosphate homeostasis gene <i>pho1</i> is actively repressed during growth in phosphate-rich medium by transcription in <i>cis</i> of a long noncoding (lnc) RNA from the 5' flanking <i>prt(nc-pho1)</i> gene. Pho1 expression is: (i) derepressed by genetic maneuvers that favor precocious lncRNA 3'-processing and termination, in response to DSR and PAS signals in <i>prt</i>; and (ii) hyperrepressed in genetic backgrounds that dampen 3'-processing/termination efficiency. Governors of 3'-processing/termination include the RNA polymerase CTD code, the CPF (cleavage and polyadenylation factor) complex, termination factors Seb1 and Rhn1, and the inositol pyrophosphate signaling molecule 1,5-IP₈ Here, we present genetic and biochemical evidence that fission yeast Duf89, a m2023-01-01T00:00:00Z2023 Jun2026-07-15T14:55:49.555Z2026-07-06T03:09:21.443ZS-EPMC101876683688229610.1261/rna.079595.123